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First published online November 3, 2003
doi: 10.1242/10.1242/dev.00806

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Development 130, 5989-5999 (2003)
Copyright © 2003 The Company of Biologists Limited

Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion

Mihye Lee1,2,*, Seungbok Lee1,3,*, Alireza Dehghani Zadeh1,* and Peter A. Kolodziej1,{dagger}

1 Department of Cell and Developmental Biology, Center for Molecular Neuroscience, Program in Developmental Biology, Vanderbilt University Medical Center, Nashville TN 37232-2175, USA
2 School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-742, Republic of Korea
3 College of Dentistry, Seoul National University, Seoul 110-740, Republic of Korea

{dagger} Author for correspondence (e-mail: kolodzp{at}ctrvax.vanderbilt.edu)

Accepted 20 August 2003

We have investigated how E-cadherin controls the elaboration of adherens junction associated cytoskeletal structures crucial for assembling tubular networks. During Drosophila development, tracheal branches are joined at branch tips through lumens that traverse doughnut-shaped fusion cells. Fusion cells form E-cadherin contacts associated with a track that contains F-actin, microtubules, and Shot, a plakin that binds F-actin and microtubules. Live imaging reveals that fusion occurs as the fusion cell apical surfaces meet after invaginating along the track. Initial track assembly requires E-cadherin binding to ß-catenin. Surprisingly, E-cadherin also controls track maturation via a juxtamembrane site in the cytoplasmic domain. Fusion cells expressing an E-cadherin mutant in this site form incomplete tracks that contain F-actin and Shot, but lack microtubules. These results indicate that E-cadherin controls track initiation and maturation using distinct, evolutionarily conserved signals to F-actin and microtubules, and employs Shot to promote adherens junction-associated cytoskeletal assembly.

Key words: E-cadherin, Drosophila, Cytoskeleton




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© The Company of Biologists Ltd 2003