spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 5 November 2003
doi: 10.1242/dev.00875

Development 130, 6187-6199 (2003)
Published by The Company of Biologists 2003
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.00875v1
130/25/6187    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gering, M.
Right arrow Articles by Patient, R. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gering, M.
Right arrow Articles by Patient, R. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Lmo2 and Scl/Tal1 convert non-axial mesoderm into haemangioblasts which differentiate into endothelial cells in the absence of Gata1

Martin Gering1, Yoshihiro Yamada2,*, Terence H. Rabbitts2 and Roger K. Patient1,{dagger}

1 Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK
2 Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

{dagger} Author for correspondence (e-mail: roger.patient{at}nottingham.ac.uk)

Accepted 16 September 2003

The LIM domain protein Lmo2 and the basic helix-loop-helix transcription factor Scl/Tal1 are expressed in early haematopoietic and endothelial progenitors and interact with each other in haematopoietic cells. While loss-of-function studies have shown that Lmo2 and Scl/Tal1 are essential for haematopoiesis and angiogenic remodelling of the vasculature, gain-of-function studies have suggested an earlier role for Scl/Tal1 in the specification of haemangioblasts, putative bipotential precursors of blood and endothelium. In zebrafish embryos, Scl/Tal1 can induce these progenitors from early mesoderm mainly at the expense of the somitic paraxial mesoderm. We show that this restriction to the somitic paraxial mesoderm correlates well with the ability of Scl/Tal1 to induce ectopic expression of its interaction partner Lmo2. Co-injection of lmo2 mRNA with scl/tal1 dramatically extends its effect to head, heart, pronephros and pronephric duct mesoderm inducing early blood and endothelial genes all along the anteroposterior axis. Erythroid development, however, is expanded only into pronephric mesoderm, remaining excluded from head, heart and somitic paraxial mesoderm territories. This restriction correlates well with activation of gata1 transcription and co-injection of gata1 mRNA along with scl/tal1 and lmo2 induces erythropoiesis more broadly without ventralising or posteriorising the embryo. While no ectopic myeloid development from the Scl/Tal1-Lmo2-induced haemangioblasts was observed, a dramatic increase in the number of endothelial cells was found. These results suggest that, in the absence of inducers of erythroid or myeloid haematopoiesis, Scl/Tal1-Lmo2-induced haemangioblasts differentiate into endothelial cells.

Key words: Haematopoiesis, Blood, Erythropoiesis, Myelopoiesis, Vasculogenesis, Pronephric duct, Heart, Lateral mesoderm, Somitic mesoderm


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S.-W. Jin and C. Patterson
The Opening Act: Vasculogenesis and the Origins of Circulation
Arterioscler. Thromb. Vasc. Biol., May 1, 2009; 29(5): 623 - 629.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Peterkin, A. Gibson, and R. Patient
Common genetic control of haemangioblast and cardiac development in zebrafish
Development, May 1, 2009; 136(9): 1465 - 1474.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
T. Cimato, J. Beers, S. Ding, M. Ma, J. P. McCoy, M. Boehm, and E. G. Nabel
Neuropilin-1 Identifies Endothelial Precursors in Human and Murine Embryonic Stem Cells Before CD34 Expression
Circulation, April 28, 2009; 119(16): 2170 - 2178.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
F. Liu and R. Patient
Genome-Wide Analysis of the Zebrafish ETS Family Identifies Three Genes Required for Hemangioblast Differentiation or Angiogenesis
Circ. Res., November 7, 2008; 103(10): 1147 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
H.-T. Huang and L.I. Zon
Regulation of Stem Cells in the Zebra Fish Hematopoietic System
Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.029v1.
[Abstract] [PDF]

Home page
 DevelopmentHome page
S. P. Mudumana, D. Hentschel, Y. Liu, A. Vasilyev, and I. A. Drummond
odd skipped related1 reveals a novel role for endoderm in regulating kidney versus vascular cell fate
Development, October 15, 2008; 135(20): 3355 - 3367.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J.-W. Xiong, Q. Yu, J. Zhang, and J. D. Mably
An Acyltransferase Controls the Generation of Hematopoietic and Endothelial Lineages in Zebrafish
Circ. Res., May 9, 2008; 102(9): 1057 - 1064.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Sumanas, G. Gomez, Y. Zhao, C. Park, K. Choi, and S. Lin
Interplay among Etsrp/ER71, Scl, and Alk8 signaling controls endothelial and myeloid cell formation
Blood, May 1, 2008; 111(9): 4500 - 4510.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Carradice and G. J. Lieschke
Zebrafish in hematology: sushi or science?
Blood, April 1, 2008; 111(7): 3331 - 3342.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Cermenati, S. Moleri, S. Cimbro, P. Corti, L. Del Giacco, R. Amodeo, E. Dejana, P. Koopman, F. Cotelli, and M. Beltrame
Sox18 and Sox7 play redundant roles in vascular development
Blood, March 1, 2008; 111(5): 2657 - 2666.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. Kobayashi and M. Yamamoto
Regulation of GATA1 Gene Expression
J. Biochem., July 1, 2007; 142(1): 1 - 10.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Z. Xu, X. Meng, Y. Cai, H. Liang, L. Nagarajan, and S. J. Brandt
Single-stranded DNA-binding proteins regulate the abundance of LIM domain and LIM domain-binding proteins
Genes & Dev., April 15, 2007; 21(8): 942 - 955.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Deleuze, E. Chalhoub, R. El-Hajj, C. Dohet, M. Le Clech, P.-O. Couraud, P. Huber, and D. Mathieu
TAL-1/SCL and Its Partners E47 and LMO2 Up-Regulate VE-Cadherin Expression in Endothelial Cells
Mol. Cell. Biol., April 1, 2007; 27(7): 2687 - 2697.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. J. Patterson, M. Gering, C. E. Eckfeldt, A. R. Green, C. M. Verfaillie, S. C. Ekker, and R. Patient
The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish
Blood, March 15, 2007; 109(6): 2389 - 2398.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Ema, T. Yokomizo, A. Wakamatsu, T. Terunuma, M. Yamamoto, and S. Takahashi
Primitive erythropoiesis from mesodermal precursors expressing VE-cadherin, PECAM-1, Tie2, endoglin, and CD34 in the mouse embryo
Blood, December 15, 2006; 108(13): 4018 - 4024.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. Alt, O. A. Elsalini, P. Schrumpf, N. Haufs, N. D. Lawson, G. C. Schwabe, S. Mundlos, A. Gruters, H. Krude, and K. B. Rohr
Arteries define the position of the thyroid gland during its developmental relocalisation.
Development, October 1, 2006; 133(19): 3797 - 3804.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
X. Li, J.-W. Xiong, C. S. Shelley, H. Park, and M. A. Arnaout
The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells
Development, September 15, 2006; 133(18): 3641 - 3650.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Gupta, H. Zhu, L. I. Zon, and T. Evans
BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis
Development, June 1, 2006; 133(11): 2177 - 2187.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
H. Priddle, D. R. E. Jones, P. W. Burridge, and R. Patient
Hematopoiesis from Human Embryonic Stem Cells: Overcoming the Immune Barrier in Stem Cell Therapies
Stem Cells, April 1, 2006; 24(4): 815 - 824.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Ema, S. Takahashi, and J. Rossant
Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors
Blood, January 1, 2006; 107(1): 111 - 117.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. Juarez, F. Su, S. Chun, M. J. Kiel, and S. E. Lyons
Distinct Roles for SCL in Erythroid Specification and Maturation in Zebrafish
J. Biol. Chem., December 16, 2005; 280(50): 41636 - 41644.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. H. Schuh, A. J. Tipping, A. J. Clark, I. Hamlett, B. Guyot, F. J. Iborra, P. Rodriguez, J. Strouboulis, T. Enver, P. Vyas, et al.
ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis
Mol. Cell. Biol., December 1, 2005; 25(23): 10235 - 10250.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. Saito, Y. Tabata, A. Muto, K.-i. Arai, and S. Watanabe
Melk-Like Kinase Plays a Role in Hematopoiesis in the Zebra Fish
Mol. Cell. Biol., August 1, 2005; 25(15): 6682 - 6693.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. J. Weber, S. E. Choe, K. A. Dooley, N. N. Paffett-Lugassy, Y. Zhou, and L. I. Zon
Mutant-specific gene programs in the zebrafish
Blood, July 15, 2005; 106(2): 521 - 530.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. L. D'Souza, A. G. Elefanty, and G. Keller
SCL/Tal-1 is essential for hematopoietic commitment of the hemangioblast but not for its development
Blood, May 15, 2005; 105(10): 3862 - 3870.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. J. Patterson, M. Gering, and R. Patient
Scl is required for dorsal aorta as well as blood formation in zebrafish embryos
Blood, May 1, 2005; 105(9): 3502 - 3511.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. M. Schlaeger, A. Schuh, S. Flitter, A. Fisher, H. Mikkola, S. H. Orkin, P. Vyas, and C. Porcher
Decoding Hematopoietic Specificity in the Helix-Loop-Helix Domain of the Transcription Factor SCL/Tal-1
Mol. Cell. Biol., September 1, 2004; 24(17): 7491 - 7502.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003