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First published online December 1, 2003
doi: 10.1242/10.1242/dev.00872

Development 130, 6545-6553 (2003)
Published by The Company of Biologists 2003
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Mitf expression is sufficient to direct differentiation of medaka blastula derived stem cells to melanocytes

Julia Béjar, Yunhan Hong* and Manfred Schartl{dagger}

Physiological Chemistry I, Biocenter of the University Würzburg, D-97074 Würzburg, Germany

{dagger} Author for correspondence (e-mail: phch1{at}biozentrum.uni-wuerzburg.de)

Accepted 16 September 2003

Embryonic stem (ES) cell lines have provided very useful models to analyse differentiation processes. We present here the development of a differentiation system using ES-like cell lines from medaka. These cells were transfected with the melanocyte specific isoform of the microphtalmia-related transcription factor (Mitf). Mitf is a basic helix-loop-helix-leucine zipper transcription factor whose M isoform is restricted to neural crest derived melanocytes and is essential for the development of these cells in vertebrates from mammals to fish. What is not clear yet is whether Mitf is a downstream factor or a master regulator of melanocyte commitment and differentiation. Expression of Mitf in the ES-like cells from medaka led to the induction of cells that, by morphology, physiology and gene expression pattern, were confirmed to be fully differentiated pigment cells. Mitf expression is therefore sufficient for the proper differentiation of medaka pluripotent stem cells into melanocytes.

Key words: Cell differentiation, ES cells, Medaka fish, Melanocyte, Mitf


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