spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

doi: 10.1242/10.1242/dev.00227

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, Q.
Right arrow Articles by Zhou, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, Q.
Right arrow Articles by Zhou, J.
Development 130, 519-526 (2003)
Copyright © 2003 The Company of Biologists Limited

The promoter targeting sequence facilitates and restricts a distant enhancer to a single promoter in the Drosophila embryo

Qing Lin, Di Wu and Jumin Zhou*

Molecular Genetics Division, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA

* Author for correspondence (e-mail: zhouj{at}wistar.upenn.edu)

Accepted 21 October 2002

Transcriptional enhancers in large gene complexes activate promoters over huge distances, yet little is known about the mechanism of these long-range interactions. We report that the promoter targeting sequence (PTS) from the Abdominal-B locus of the Drosophila bithorax complex facilitates the activity of a distantly located enhancer in transgenic embryos and that it restricts the enhancer to a single promoter. These functions are heritable in all successive generations. We also show that the PTS functions only when itself and an insulator are located between the enhancer and the promoter. These findings suggest that the PTS may facilitate long-range enhancer-promoter interactions in the endogenous Abdominal-B locus. We propose that the PTS establishes a stable chromatin structure between an enhancer and a promoter, which facilitates yet restricts an enhancer to a single promoter.

Key words: Abdominal-B, Insulator, PTS, Fab-8, suHw, IAB5




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
O. Kyrchanova, S. Toshchakov, Y. Podstreshnaya, A. Parshikov, and P. Georgiev
Functional Interaction between the Fab-7 and Fab-8 Boundaries and the Upstream Promoter Region in the Drosophila Abd-B Gene
Mol. Cell. Biol., June 15, 2008; 28(12): 4188 - 4195.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Chetverina, E. Savitskaya, O. Maksimenko, L. Melnikova, O. Zaytseva, A. Parshikov, A. V. Galkin, and P. Georgiev
Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs
Nucleic Acids Res., February 11, 2008; 36(3): 929 - 937.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
O. Kyrchanova, S. Toshchakov, A. Parshikov, and P. Georgiev
Study of the Functional Interaction between Mcp Insulators from the Drosophila bithorax Complex: Effects of Insulator Pairing on Enhancer-Promoter Communication
Mol. Cell. Biol., April 15, 2007; 27(8): 3035 - 3043.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Q. Lin, Q. Chen, L. Lin, S. Smith, and J. Zhou
Promoter targeting sequence mediates enhancer interference in the Drosophila embryo
PNAS, February 27, 2007; 104(9): 3237 - 3242.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. M. Lee and C.-t. Wu
Enhancer-Promoter Communication at the yellow Gene of Drosophila melanogaster: Diverse Promoters Participate in and Regulate trans Interactions
Genetics, December 1, 2006; 174(4): 1867 - 1880.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. Dorsett, J. C. Eissenberg, Z. Misulovin, A. Martens, B. Redding, and K. McKim
Effects of sister chromatid cohesion proteins on cut gene expression during wing development in Drosophila
Development, November 1, 2005; 132(21): 4743 - 4753.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Gruzdeva, O. Kyrchanova, A. Parshikov, A. Kullyev, and P. Georgiev
The Mcp Element from the bithorax Complex Contains an Insulator That Is Capable of Pairwise Interactions and Can Facilitate Enhancer-Promoter Communication
Mol. Cell. Biol., May 1, 2005; 25(9): 3682 - 3689.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. G. West and P. Fraser
Remote control of gene transcription
Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R101 - R111.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Q. Lin, Q. Chen, L. Lin, and J. Zhou
The Promoter Targeting Sequence mediates epigenetically heritable transcription memory
Genes & Dev., November 1, 2004; 18(21): 2639 - 2651.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Melnikova, F. Juge, N. Gruzdeva, A. Mazur, G. Cavalli, and P. Georgiev
Interaction between the GAGA factor and Mod(mdg4) proteins promotes insulator bypass in Drosophila
PNAS, October 12, 2004; 101(41): 14806 - 14811.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. A. Rollins, M. Korom, N. Aulner, A. Martens, and D. Dorsett
Drosophila Nipped-B Protein Supports Sister Chromatid Cohesion and Opposes the Stromalin/Scc3 Cohesion Factor To Facilitate Long-Range Activation of the cut Gene
Mol. Cell. Biol., April 15, 2004; 24(8): 3100 - 3111.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003