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doi: 10.1242/10.1242/dev.00297

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Development 130, 719-728 (2003)
Copyright © 2003 The Company of Biologists Limited

Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems

Qing-Xin Liu1,*, Marek Jindra1,2,*, Hitoshi Ueda1, Yasushi Hiromi1 and Susumu Hirose1,{dagger}

1 Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411-8540, Japan
2 Institute of Entomology, Czech Academy of Sciences, Ceske Budejovice, 37005 Czech Republic

{dagger} Author for correspondence (e-mail: shirose{at}lab.nig.ac.jp)

Accepted 19 November 2002

During gene activation, the effect of binding of transcription factors to cis-acting DNA sequences is transmitted to RNA polymerase by means of co-activators. Although co-activators contribute to the efficiency of transcription, their developmental roles are poorly understood. We used Drosophila to conduct molecular and genetic dissection of an evolutionarily conserved but unique co-activator, Multiprotein Bridging Factor 1 (MBF1), in a multicellular organism. Through immunoprecipitation, MBF1 was found to form a ternary complex including MBF1, TATA-binding protein (TBP) and the bZIP protein Tracheae Defective (TDF)/Apontic. We have isolated a Drosophila mutant that lacks the mbf1 gene in which no stable association between TBP and TDF is detectable, and transcription of a TDF-dependent reporter gene is reduced by 80%. Although the null mutants of mbf1 are viable, tdf becomes haploinsufficient in mbf1-deficient background, causing severe lesions in tracheae and the central nervous system, similar to those resulting from a complete loss of tdf function. These data demonstrate a crucial role of MBF1 in the development of tracheae and central nervous system.

Key words: TDF, MBF1, Co-activator, Trachea, CNS, Drosophila




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