|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00290
Section of Cell and Developmental Biology, U.C. San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116, USA
* Author for correspondence (e-mail: lsmith{at}biomail.ucsd.edu)
Accepted 29 October 2002
We have taken a genetic approach to investigating cytoskeleton-dependent mechanisms governing cell morphogenesis in the maize leaf epidermis. Previously, we showed that the Brick1 (Brk1) gene is required for the formation of epidermal cell lobes as well as for properly polarized divisions of stomatal subsidiary mother cells, and encodes an 8 kDa protein highly conserved in plants and animals. Here, we show that two additional Brick genes, Brk2 and Brk3, are involved in the same aspects of epidermal cell morphogenesis and division. As shown previously for Brk1, analysis of the cytoskeleton shows that Brk2 and Brk3 are required for the formation of local F-actin enrichments associated with lobe outgrowth in wild-type cells. Analysis of brk1;brk2, brk1;brk3 and brk2;brk3 double mutants shows that their phenotypes are the same as those of brk single mutants. Mosaic analysis shows that Brk1 acts non cell-autonomously over a short distance. By contrast, Brk2 and Brk3 act cell-autonomously to promote pavement cell lobe formation, but Brk3 acts non cell-autonomously, and Brk2 partially non cell-autonomously, to promote polarized subsidiary mother cell divisions. Together, these observations indicate that all three Brk genes act in a common pathway in which each Brk gene has a distinct function. Recent work demonstrating a function for the mammalian homolog of BRK1 (HSPC300) in activation of Arp2/3-dependent actin polymerization implicates the Brk pathway in local regulation of actin polymerization in plant cells.
Key words: Cell polarity, Cell morphogenesis, Maize leaf development, Mosaic analysis, BRICK1, HSPC300
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. J. Wright, K. Gallagher, and L. G. Smith discordia1 and alternative discordia1 Function Redundantly at the Cortical Division Site to Promote Preprophase Band Formation and Orient Division Planes in Maize PLANT CELL, January 1, 2009; 21(1): 234 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dyachok, M.-R. Shao, K. Vaughn, A. Bowling, M. Facette, S. Djakovic, L. Clark, and L. Smith Plasma Membrane-Associated SCAR Complex Subunits Promote Cortical F-Actin Accumulation and Normal Growth Characteristics in Arabidopsis Roots Mol Plant, November 1, 2008; 1(6): 990 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ringli, L. Bigler, B. M. Kuhn, R.-M. Leiber, A. Diet, D. Santelia, B. Frey, S. Pollmann, and M. Klein The Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape Formation PLANT CELL, June 1, 2008; 20(6): 1470 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-F. Perroud and R. S. Quatrano BRICK1 Is Required for Apical Cell Growth in Filaments of the Moss Physcomitrella patens but Not for Gametophore Morphology PLANT CELL, February 1, 2008; 20(2): 411 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Guimil and C. Dunand Cell growth and differentiation in Arabidopsis epidermal cells J. Exp. Bot., November 1, 2007; 58(14): 3829 - 3840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Uhrig, M. Mutondo, I. Zimmermann, M. J. Deeks, L. M. Machesky, P. Thomas, S. Uhrig, C. Rambke, P. J. Hussey, and M. Hulskamp The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis Development, March 1, 2007; 134(5): 967 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bannigan, A. M. D. Wiedemeier, R. E. Williamson, R. L. Overall, and T. I. Baskin Cortical Microtubule Arrays Lose Uniform Alignment Between Cells and are Oryzalin Resistant in the Arabidopsis Mutant, radially swollen 6 Plant Cell Physiol., July 1, 2006; 47(7): 949 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Djakovic, J. Dyachok, M. Burke, M. J. Frank, and L. G. Smith BRICK1/HSPC300 functions with SCAR and the ARP2/3 complex to regulate epidermal cell shape in Arabidopsis Development, March 15, 2006; 133(6): 1091 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, J. Dyachok, S. Krishnakumar, L. G. Smith, and D. G. Oppenheimer IRREGULAR TRICHOME BRANCH1 in Arabidopsis Encodes a Plant Homolog of the Actin-Related Protein2/3 Complex Activator Scar/WAVE That Regulates Actin and Microtubule Organization PLANT CELL, August 1, 2005; 17(8): 2314 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhong, D. H. Burk, C. J. Nairn, A. Wood-Jones, W. H. Morrison III, and Z.-H. Ye Mutation of SAC1, an Arabidopsis SAC Domain Phosphoinositide Phosphatase, Causes Alterations in Cell Morphogenesis, Cell Wall Synthesis, and Actin Organization PLANT CELL, May 1, 2005; 17(5): 1449 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhong, D. H. Burk, W. H. Morrison III, and Z.-H. Ye FRAGILE FIBER3, an Arabidopsis Gene Encoding a Type II Inositol Polyphosphate 5-Phosphatase, Is Required for Secondary Wall Synthesis and Actin Organization in Fiber Cells PLANT CELL, December 1, 2004; 16(12): 3242 - 3259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, K. Sorefan, G. Hemmann, and M. W. Bevan Arabidopsis NAP and PIR Regulate Actin-Based Cell Morphogenesis and Multiple Developmental Processes Plant Physiology, November 1, 2004; 136(3): 3616 - 3627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, L. Blanchoin, Z. Yang, and E. M. Lord The Putative Arabidopsis Arp2/3 Complex Controls Leaf Cell Morphogenesis Plant Physiology, August 1, 2003; 132(4): 2034 - 2044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mathur, N. Mathur, V. Kirik, B. Kernebeck, B. P. Srinivas, and M. Hulskamp Arabidopsis CROOKED encodes for the smallest subunit of the ARP2/3 complex and controls cell shape by region specific fine F-actin formation Development, July 15, 2003; 130(14): 3137 - 3146. [Abstract] [Full Text] [PDF]
|
|
