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doi: 10.1242/10.1242/dev.00314
,
1 Institute of Neuroscience, University of Oregon, Eugene, OR 97403-1254,
USA
2 Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY,
UK
* Present address: Developmental Genetics Program and Department of Cell
Biology, Skirball Institute of Biomolecular Medicine, New York University
School of Medicine, New York, NY 10016, USA
Present address: Department of Anatomy, University of Cambridge, Downing
Street, Cambridge CB2 3DY, UK
Author for correspondence (e-mail:
rja46{at}cam.ac.uk)
Accepted 18 November 2002
Promptly after the notochord domain is specified in the vertebrate dorsal mesoderm, it undergoes dramatic morphogenesis. Beginning during gastrulation, convergence and extension movements change a squat cellular array into a narrow, elongated one that defines the primary axis of the embryo. Convergence and extension might be coupled by a highly organized cellular intermixing known as mediolateral intercalation behavior (MIB). To learn whether MIB drives early morphogenesis of the zebrafish notochord, we made 4D recordings and quantitatively analyzed both local cellular interactions and global changes in the shape of the dorsal mesodermal field. We show that MIB appears to mediate convergence and can account for extension throughout the dorsal mesoderm. Comparing the notochord and adjacent somitic mesoderm reveals that extension can be regulated separately from convergence. Moreover, mutational analysis shows that extension does not require convergence. Hence, a cellular machine separate from MIB that can drive dorsal mesodermal extension exists in the zebrafish gastrula. The likely redundant control of morphogenesis may provide for plasticity at this critical stage of early development.
Key words: Gradients, Morphogenesis, no tail, Mediolateral intercalation behavior, Notochord, Convergence, Extension, Gastrulation, Epiboly, Zebrafish
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