QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

doi: 10.1242/10.1242/dev.00315

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Máthé, E. Articles by Glover, D. M. Search for Related Content PubMed PubMed Citation Articles by Máthé, E. Articles by Glover, D. M. Social Bookmarking                         What's this?

Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis

¹ Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EN, UK
² Drosophila Genetic Resource Center, Kyoto Institute of Technology, Sagaippongi-cho, Ukyou-ku, Kyoto, 616-8354, Japan

^* Author for correspondence (e-mail: dmg25{at}mole.bio.cam.ac.uk)

Accepted 29 November 2002

Drosophila oocyte differentiation is preceded by the formation of a polarised 16-cell cyst from a single progenitor stem cell as a result of four rounds of asymmetric mitosis followed by incomplete cytokinesis. We show that the Orbit/Mast microtubule-associated protein is required at several stages in the formation of such polarised 16-cell cysts. In wild-type cysts, the Orbit/Mast protein not only associates with the mitotic spindle and its poles, but also with the central spindle (spindle remnant), ring canal and fusome, suggesting it participates in interactions between these structures. In orbit mutants, the stem cells and their associated fusomes are eventually lost as Orbit/Mast protein is depleted. The mitotic spindles of those cystocytes that do divide are either diminutive or monopolar, and do not make contact with the fusome. Moreover, the spindle remnants and ring canals fail to differentiate correctly in such cells and the structure of fusome is compromised. The Orbit/Mast protein thus appears to facilitate multiple interactions of the fusome with mitotic spindles and ring canals. This ensures correct growth of the fusome into a branched asymmetrically distributed organelle that is pre-determinative of 16-cell cyst formation and oocyte fate specification. Finally the Orbit/Mast protein is required during mid-oogenesis for the organisation of the polarised microtubule network inside the 16-cell cyst that ensures oocyte differentiation. The localisation of CLIP-190 to such microtubules and to the fusome is dependent upon Orbit/Mast to which it is complexed.

Key words: Orbit, Mast, CLIP-190, Anillin, Pavarotti-KLP, Fusome, Asymmetric division, CLASP, Cytokinesis, Oogenesis, Drosophila

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				R. Reis, T. Feijao, S. Gouveia, A. J. Pereira, I. Matos, P. Sampaio, H. Maiato, and C. E. Sunkel Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics J. Cell Sci., July 15, 2009; 122(14): 2543 - 2553. [Abstract] [Full Text] [PDF]

				V. Kirik, U. Herrmann, C. Parupalli, J. C. Sedbrook, D. W. Ehrhardt, and M. Hulskamp CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis J. Cell Sci., December 15, 2007; 120(24): 4416 - 4425. [Abstract] [Full Text] [PDF]

				A. Grallert, C. Beuter, R. A. Craven, S. Bagley, D. Wilks, U. Fleig, and I. M. Hagan S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner Genes & Dev., September 1, 2006; 20(17): 2421 - 2436. [Abstract] [Full Text] [PDF]

				Y. Mimori-Kiyosue and S. Tsukita "Search-and-Capture" of Microtubules through Plus-End-Binding Proteins (+TIPs) J. Biochem., September 1, 2003; 134(3): 321 - 326. [Abstract] [Full Text] [PDF]