QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

doi: 10.1242/10.1242/dev.00356

This Article Figures Only Full Text Full Text (PDF) Supplemental Data Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ang, L.-H. Articles by Hing, H. Search for Related Content PubMed PubMed Citation Articles by Ang, L.-H. Articles by Hing, H. Social Bookmarking                         What's this?

Dock and Pak regulate olfactory axon pathfinding in Drosophila

Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, 601 South Goodwin Avenue, C626, Urbana, IL 61801, USA

^* Author for correspondence (e-mail: hing{at}life.uiuc.edu)

Accepted 19 December 2002

The convergence of olfactory axons expressing particular odorant receptor (Or) genes on spatially invariant glomeruli in the brain is one of the most dramatic examples of precise axon targeting in developmental neurobiology. The cellular and molecular mechanisms by which olfactory axons pathfind to their targets are poorly understood. We report here that the SH2/SH3 adapter Dock and the serine/threonine kinase Pak are necessary for the precise guidance of olfactory axons. Using antibody localization, mosaic analyses and cell-type specific rescue, we observed that Dock and Pak are expressed in olfactory axons and function autonomously in olfactory neurons to regulate the precise wiring of the olfactory map. Detailed analyses of the mutant phenotypes in whole mutants and in small multicellular clones indicate that Dock and Pak do not control olfactory neuron (ON) differentiation, but specifically regulate multiple aspects of axon trajectories to guide them to their cognate glomeruli. Structure/function studies show that Dock and Pak form a signaling pathway that mediates the response of olfactory axons to guidance cues in the developing antennal lobe (AL). Our findings therefore identify a central signaling module that is used by ONs to project to their cognate glomeruli.

Key words: Drosophila, Dock, Pak, Olfactory, Axon Pathfinding, Signaling, Antennal lobe, Glomeruli

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				L. Pan and K. S. Broadie Drosophila Fragile X Mental Retardation Protein and Metabotropic Glutamate Receptor A Convergently Regulate the Synaptic Ratio of Ionotropic Glutamate Receptor Subclasses J. Neurosci., November 7, 2007; 27(45): 12378 - 12389. [Abstract] [Full Text] [PDF]

				J. Rohrbough, E. Rushton, E. Woodruff III, T. Fergestad, K. Vigneswaran, and K. Broadie Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation Genes & Dev., October 15, 2007; 21(20): 2607 - 2628. [Abstract] [Full Text] [PDF]

				K. F. Stortkuhl, R. Kettler, S. Fischer, and B. T. Hovemann An Increased Receptive Field of Olfactory Receptor Or43a in the Antennal Lobe of Drosophila Reduces Benzaldehyde-driven Avoidance Behavior Chem Senses, January 1, 2005; 30(1): 81 - 87. [Abstract] [Full Text] [PDF]

				C. Hofmann, M. Shepelev, and J. Chernoff The genetics of Pak J. Cell Sci., September 1, 2004; 117(19): 4343 - 4354. [Abstract] [Full Text] [PDF]

				W. Li and K.-L. Guan The Down Syndrome Cell Adhesion Molecule (DSCAM) Interacts with and Activates Pak J. Biol. Chem., July 30, 2004; 279(31): 32824 - 32831. [Abstract] [Full Text] [PDF]

				D. Jhaveri, S. Saharan, A. Sen, and V. Rodrigues Positioning sensory terminals in the olfactory lobe of Drosophila by Robo signaling Development, May 1, 2004; 131(9): 1903 - 1912. [Abstract] [Full Text] [PDF]