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First published online 3 December 2003
doi: 10.1242/dev.00921
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,
1 Department of Molecular and Cellular Biology, Harvard University, Cambridge,
MA 02138, USA
2 Department of Pathology, Massachusetts General Hospital, Charlestown,
Massachusetts 02129, USA
Author for correspondence (e-mail:
dmelton{at}biohp.harvard.edu)
Accepted 30 September 2003
To define genetic pathways that regulate development of the endocrine
pancreas, we generated transcriptional profiles of enriched cells isolated
from four biologically significant stages of endocrine pancreas development:
endoderm before pancreas specification, early pancreatic progenitor cells,
endocrine progenitor cells and adult islets of Langerhans. These analyses
implicate new signaling pathways in endocrine pancreas development, and
identified sets of known and novel genes that are temporally regulated, as
well as genes that spatially define developing endocrine cells from their
neighbors. The differential expression of several genes from each time point
was verified by RT-PCR and in situ hybridization. Moreover, we present
preliminary functional evidence suggesting that one transcription factor
encoding gene (Myt1), which was identified in our screen, is
expressed in endocrine progenitors and may regulate 
, ß and
cell development. In addition to identifying new genes that regulate
endocrine cell fate, this global gene expression analysis has uncovered
informative biological trends that occur during endocrine differentiation.
Key words: Myt1, endoderm, Pancreas, Endocrine, Islets, Microarray
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