QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online May 5, 2004
doi: 10.1242/10.1242/dev.01118

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ng, S.-C. Articles by Martelli, P. Search for Related Content PubMed PubMed Citation Articles by Ng, S.-C. Articles by Martelli, P.

The first cell cycle after transfer of somatic cell nuclei in a non-human primate

Soon-Chye Ng^*,, Naiqing Chen^*, Wan-Yue Yip^*, Swee-Lian Liow^*, Guo-Qing Tong, Barbara Martelli, Lay Geok Tan and Paolo Martelli

Laboratory of Nuclear Reprogramming and Cell Differentiation, Department of Obstetrics and Gynaecology, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore

Author for correspondence (e-mail: scng{at}embryonics.biz)

Accepted 11 February 2004

Production of genetically identical non-human primates through somatic cell nuclear transfer (SCNT) can provide diseased genotypes for research and clarify embryonic stem cell potentials. Understanding the cellular and molecular changes in SCNT is crucial to its success. Thus the changes in the first cell cycle of reconstructed zygotes after nuclear transfer (NT) of somatic cells in the Long-tailed Macaque (Macaca fascicularis) were studied. Embryos were reconstructed by injecting cumulus and fibroblasts from M. fascicularis and M. silenus, into enucleated M. fascicularis oocytes. A spindle of unduplicated premature condensed chromosome (PCC spindle) from the donor somatic cell was formed at 2 hours after NT. Following activation, the chromosomes segregated and moved towards the two PCC spindle poles, then formed two nuclei. Twenty-four hours after activation, the first cell division occurred. A schematic of the first cell cycle changes following injection of a somatic cell into an enucleated oocyte is proposed. Ninety-three reconstructed embryos were transferred into 31 recipients, resulting in 7 pregnancies that were confirmed by ultrasound; unfortunately none progressed beyond 60 days.

Key words: Somatic cell nuclear transfer, Cell cycle, Spindle formation, Non-human primate, Embryo transfer

Related articles in Development:

Primate cloning: not a spindle problem

Development 2004 131: e1006. [Full Text]  

This article has been cited by other articles:

				S.M. Mitalipov, Q. Zhou, J.A. Byrne, W.Z. Ji, R.B. Norgren, and D.P. Wolf Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling Hum. Reprod., August 1, 2007; 22(8): 2232 - 2242. [Abstract] [Full Text] [PDF]

				J. Yang, S. Yang, N. Beaujean, Y. Niu, X. He, Y. Xie, X. Tang, L. Wang, Q. Zhou, and W. Ji Epigenetic Marks in Cloned Rhesus Monkey Embryos: Comparison with Counterparts Produced In Vitro Biol Reprod, January 1, 2007; 76(1): 36 - 42. [Abstract] [Full Text] [PDF]

				Q. Zhou, S.H. Yang, C.H. Ding, X.C. He, Y.H. Xie, T.B. Hildebrandt, S.M. Mitalipov, X.H. Tang, D.P. Wolf, and W.Z. Ji A comparative approach to somatic cell nuclear transfer in the rhesus monkey Hum. Reprod., October 1, 2006; 21(10): 2564 - 2571. [Abstract] [Full Text] [PDF]