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First published online 5 May 2004
doi: 10.1242/dev.01141
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1 Department of Zoology, University of Toronto, 25 Harbord Street, Toronto,
Ontario M5S 3G5, Canada
2 Department of Biochemistry, Boston University School of Medicine, 715 Albany
Street, Boston, MA 02118, USA
* Author for correspondence (e-mail: winklbauer{at}zoo.utoronto.ca)
Accepted 16 February 2004
In vertebrates, PDGFA and its receptor, PDGFR
, are expressed in the
early embryo. Impairing their function causes an array of developmental
defects, but the underlying target processes that are directly controlled by
these factors are not well known. We show that in the Xenopus
gastrula, PDGFA/PDGFR signaling is required for the directional
migration of mesodermal cells on the extracellular matrix of the blastocoel
roof. Blocking PDGFR function in the mesoderm does not inhibit
migration per se, but results in movement that is randomized and no longer
directed towards the animal pole. Likewise, compromising PDGFA function in the
blastocoel roof substratum abolishes directionality of movement.
Overexpression of wild-type PDGFA, or inhibition of PDGFA both lead to
randomized migration, disorientation of polarized mesodermal cells, decreased
movement towards the animal pole, and reduced head formation and axis
elongation. This is consistent with an instructive role for PDGFA in the
guidance of mesoderm migration.
Key words: Xenopus, PDGF, Gastrulation, Cell migration
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