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First published online 2 June 2004
doi: 10.1242/dev.01185

Development 131, 3081-3091 (2004)
Published by The Company of Biologists 2004
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Organization of cardiac chamber progenitors in the zebrafish blastula

Brian R. Keegan1, Dirk Meyer2 and Deborah Yelon1,*

1 Developmental Genetics Program and Department of Cell Biology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
2 Department of Developmental Biology, Biology I, University of Freiburg, Freiburg, Germany

* Author for correspondence (e-mail: yelon{at}saturn.med.nyu.edu)

Accepted 22 March 2004

Organogenesis requires the specification of a variety of cell types and the organization of these cells into a particular three-dimensional configuration. The embryonic vertebrate heart is organized into two major chambers, the ventricle and atrium, each consisting of two tissue layers, the myocardium and endocardium. The cellular and molecular mechanisms responsible for the separation of ventricular and atrial lineages are not well understood. To test models of cardiac chamber specification, we generated a high-resolution fate map of cardiac chamber progenitors in the zebrafish embryo at 40% epiboly, a stage prior to the initiation of gastrulation. Our map reveals a distinct spatial organization of myocardial progenitors: ventricular myocardial progenitors are positioned closer to the margin and to the dorsal midline than are atrial myocardial progenitors. By contrast, ventricular and atrial endocardial progenitors are not spatially organized at this stage. The relative orientations of ventricular and atrial myocardial progenitors before and after gastrulation suggest orderly movements of these populations. Furthermore, the initial positions of myocardial progenitors at 40% epiboly indicate that signals residing at the embryonic margin could influence chamber fate assignment. Indeed, via fate mapping, we demonstrate that Nodal signaling promotes ventricular fate specification near the margin, thereby playing an important early role during myocardial patterning.

Key words: Zebrafish, Ventricle, Atrium, Myocardium, Endocardium, Fate map, Nodal


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