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First published online 14 July 2004
doi: 10.1242/dev.01247
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1 European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117
Heidelberg, Germany
2 Institute of Toxicology and Genetics, Research Center Karlsruhe, PO Box 3640,
76021 Karlsruhe, Germany
* Author for correspondence (e-mail: carl.neumann{at}embl-heidelberg.de)
Accepted 4 May 2004
Neurogenesis in the zebrafish retina occurs in several waves of differentiation. The first neurogenic wave generates ganglion cells and depends on hedgehog (hh) signaling activity. Using transgenic zebrafish embryos that express GFP under the control of the sonic hedgehog (shh) promoter, we imaged the differentiation wave in the retina and show that, in addition to the wave in the ganglion cell layer, shh expression also spreads in the inner nuclear layer. This second wave generates amacrine cells expressing shh, and although it overlaps temporally with the first wave, it does not depend on it, as it occurs in the absence of ganglion cells. We also show that differentiation of cell types found in the inner and outer nuclear layers, as well as lamination of the retina, depends on shh. By performing mosaic analysis, we demonstrate that Shh directs these events as a short-range signal within the neural retina.
Key words: Sonic hedgehog, Atonal homolog 5, Retina, Differentiation, Neurogenesis, Zebrafish
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