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First published online 27 July 2004
doi: 10.1242/dev.01289

Development 131, 4251-4261 (2004)
Published by The Company of Biologists 2004
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Differential cytoplasmic mRNA localisation adjusts pair-rule transcription factor activity to cytoarchitecture in dipteran evolution

Simon L. Bullock1,*, Michael Stauber2,*, Alexander Prell2, Julian R. Hughes1, David Ish-Horowicz1 and Urs Schmidt-Ott2,3,{dagger}

1 Cancer Research UK, Developmental Genetics Laboratory, PO Box 123, 44 Lincoln's Inn Fields, London WC2A 3PX, UK
2 Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Entwicklungsbiologie, Am Fassberg 11, 37077 Göttingen, Germany
3 University of Chicago, Department of Organismal Biology and Anatomy, CLSC 921B, 920 East 58th Street, Chicago, IL 60615, USA

{dagger} Author for correspondence (e-mail: uschmid{at}uchicago.edu)

Accepted 7 June 2004

Establishment of segmental pattern in the Drosophila syncytial blastoderm embryo depends on pair-rule transcriptional regulators. mRNA transcripts of pair-rule genes localise to the apical cytoplasm of the blastoderm via a selective dynein-based transport system and signals within their 3'-untranslated regions. However, the functional and evolutionary significance of this process remains unknown. We have analysed subcellular localisation of mRNAs from multiple dipteran species both in situ and by injection into Drosophila embryos. We find that although localisation of wingless transcripts is conserved in Diptera, localisation of even-skipped and hairy pair-rule transcripts is evolutionarily labile and correlates with taxon-specific changes in positioning of nuclei. We show in Drosophila that localised pair-rule transcripts target their proteins in close proximity to the nuclei and increase the reliability of the segmentation process by augmenting gene activity. Our data suggest that mRNA localisation signals in pair-rule transcripts affect nuclear protein uptake and thereby adjust gene activity to a variety of dipteran blastoderm cytoarchitectures.

Key words: Pair-rule, Developmental evolution, mRNA localisation, Cytoarchitecture, Diptera


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