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First published online September 1, 2004
doi: 10.1242/10.1242/dev.01321
1 Laboratory for Germline Development, RIKEN Center for Developmental Biology,
Kobe, Hyogo 650-0047, Japan
2 Okazaki Institute for Integrative Bioscience, National Institute for Basic
Biology, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787,
Japan
3 Core Research for Evolutional Science and Technology (CREST), Japan Science
and Technology Agency, Kawaguchi, Saitama 332-0012, Japan
* Authors for correspondence (e-mail: skob{at}nibb.ac.jp and akiran{at}cdb.riken.jp)
Accepted 22 June 2004
In many animals, primordial germ cells (PGCs) migrate through the embryo towards the future gonad, a process guided by attractive and repulsive cues provided from surrounding somatic cells. In Drosophila, the two related lipid phosphate phosphatases (LPPs), Wunen (Wun) and Wun2, are thought to degrade extracellular substrates and to act redundantly in somatic cells to provide a repulsive environment to steer the migration of PGCs, or pole cells. Wun and Wun2 also affect the viability of pole cells, because overexpression of either one in somatic cells causes pole cell death. However, the means by which they regulate pole cell migration and survival remains elusive. We report that Wun2 has a maternal function required for the survival of pole cells during their migration to the gonad. Maternal wun2 RNA was found to be concentrated in pole cells and pole cell-specific expression of wun2 rescued the pole cell death phenotype of the maternal wun2 mutant, suggesting that wun2 activity in pole cells is required for their survival. Furthermore, we obtained genetic evidence that pole cell survival requires a proper balance of LPP activity in pole cells and somatic cells. We propose that Wun2 in pole cells competes with somatic Wun and Wun2 for a common lipid phosphate substrate, which is required by pole cells to produce their survival signal. In somatic cells, Wun and Wun2 may provide a repulsive environment for pole cell migration by depleting this extracellular substrate.
Key words: Germ cells, Lipid phosphate phosphatase, Cell survival, Cell migration, Drosophila
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