|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 25 August 2004
doi: 10.1242/dev.01343
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA
* Author for correspondence (e-mail: pschedl{at}molbio.princeton.edu)
Accepted 8 July 2004
The Fab-7 boundary functions to ensure the autonomous activity of the iab-6 and iab-7 cis-regulatory domains in the Drosophila Bithorax Complex from early embryogenesis through to the adult stage. Although Fab-7 is required only for the proper development of a single posterior parasegment, it is active in all tissues and stages of development that have been examined. In this respect, Fab-7 resembles conventional constitutive boundaries in flies and other eukaryotes that act through ubiquitous cis-elements and trans-acting factors. Surprisingly, however, we find that the constitutive activity of Fab-7 is generated by combining sub-elements with developmentally restricted boundary function. We provide in vivo evidence that the Fab-7 boundary contains separable regions that function at different stages of development. These findings suggest that the units (domains) of genetic regulation that boundaries delimit can expand or contract by switching insulator function off or on in a temporally regulated fashion.
Key words: Fab-7, Drosophila, Bithorax, Boundary, Insulator, Abd-B
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  O. Maksimenko, A. Golovnin, and P. Georgiev Enhancer-Promoter Communication Is Regulated by Insulator Pairing in a Drosophila Model Bigenic Locus Mol. Cell. Biol., September 1, 2008; 28(17): 5469 - 5477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kyrchanova, S. Toshchakov, Y. Podstreshnaya, A. Parshikov, and P. Georgiev Functional Interaction between the Fab-7 and Fab-8 Boundaries and the Upstream Promoter Region in the Drosophila Abd-B Gene Mol. Cell. Biol., June 15, 2008; 28(12): 4188 - 4195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chetverina, E. Savitskaya, O. Maksimenko, L. Melnikova, O. Zaytseva, A. Parshikov, A. V. Galkin, and P. Georgiev Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs Nucleic Acids Res., February 11, 2008; 36(3): 929 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Aoki, S. Schweinsberg, J. Manasson, and P. Schedl A Stage-Specific Factor Confers Fab-7 Boundary Activity during Early Embryogenesis in Drosophila Mol. Cell. Biol., February 1, 2008; 28(3): 1047 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rodin, O. Kyrchanova, E. Pomerantseva, A. Parshikov, and P. Georgiev New Properties of Drosophila Fab-7 Insulator Genetics, September 1, 2007; 177(1): 113 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kyrchanova, S. Toshchakov, A. Parshikov, and P. Georgiev Study of the Functional Interaction between Mcp Insulators from the Drosophila bithorax Complex: Effects of Insulator Pairing on Enhancer-Promoter Communication Mol. Cell. Biol., April 15, 2007; 27(8): 3035 - 3043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schweinsberg, K. Hagstrom, D. Gohl, P. Schedl, R. P. Kumar, R. Mishra, and F. Karch The Enhancer-Blocking Activity of the Fab-7 Boundary From the Drosophila Bithorax Complex Requires GAGA-Factor-Binding Sites Genetics, November 1, 2004; 168(3): 1371 - 1384. [Abstract] [Full Text] [PDF]
|
|
