QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 17 December 2003
doi: 10.1242/dev.00944

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: dev.00944v1 131/2/401    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ji, J.-Y. Articles by Schubiger, G. Search for Related Content PubMed PubMed Citation Articles by Ji, J.-Y. Articles by Schubiger, G. Social Bookmarking                         What's this?

Both Cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in Drosophila

¹ Department of Biology, University of Washington, Seattle, WA 98195-1800, USA
² Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA

^* Author for correspondence (e-mail: gerold{at}u.washington.edu)

Accepted 24 October 2003

The earliest embryonic mitoses in Drosophila, as in other animals except mammals, are viewed as synchronous and of equal duration. However, we observed that total cell-cycle length steadily increases after cycle 7, solely owing to the extension of interphase. Between cycle 7 and cycle 10, this extension is DNA-replication checkpoint independent, but correlates with the onset of Cyclin B oscillation. In addition, nuclei in the middle of embryos have longer metaphase and shorter anaphase than nuclei at the two polar regions. Interestingly, sister chromatids move faster in anaphase in the middle than the posterior region. These regional differences correlate with local differences in Cyclin B concentration. After cycle 10, interphase and total cycle duration of nuclei in the middle of the embryo are longer than at the poles. Because interphase also extends in checkpoint mutant (grapes) embryo after cycle 10, although less dramatic than wild-type embryos, interphase extension after cycle 10 is probably controlled by both Cyclin B limitation and the DNA-replication checkpoint.

Key words: Early embryonic mitosis, Cdk1-CycB, Drosophila, Metaphase, Interphase

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				X. Lu, J. M. Li, O. Elemento, S. Tavazoie, and E. F. Wieschaus Coupling of zygotic transcription to mitotic control at the Drosophila mid-blastula transition Development, June 15, 2009; 136(12): 2101 - 2110. [Abstract] [Full Text] [PDF]

				M. L. McCleland, J. A. Farrell, and P. H. O'Farrell Influence of cyclin type and dose on mitotic entry and progression in the early Drosophila embryo J. Cell Biol., March 9, 2009; 184(5): 639 - 646. [Abstract] [Full Text] [PDF]

				J. A. Merkle, J. L. Rickmyre, A. Garg, E. B. Loggins, J. N. Jodoin, E. Lee, L. P. Wu, and L. A. Lee no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila Development, February 1, 2009; 136(3): 449 - 459. [Abstract] [Full Text] [PDF]

				A. Royou, D. McCusker, D. R. Kellogg, and W. Sullivan Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation J. Cell Biol., October 6, 2008; 183(1): 63 - 75. [Abstract] [Full Text] [PDF]

				J. Crest, N. Oxnard, J.-Y. Ji, and G. Schubiger Onset of the DNA Replication Checkpoint in the Early Drosophila Embryo Genetics, February 1, 2007; 175(2): 567 - 584. [Abstract] [Full Text] [PDF]

				Z. Venkei, I. Gaspar, G. Toth, and J. Szabad {alpha}4-Tubulin is involved in rapid formation of long microtubules to push apart the daughter centrosomes during earlyx Drosophila embryogenesis J. Cell Sci., August 1, 2006; 119(15): 3238 - 3248. [Abstract] [Full Text] [PDF]