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First published online 17 December 2003
doi: 10.1242/dev.00918
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1 Laboratoire de Méiose et de Maturation Gamétique, DRR / DSV/ CEA
– U566 INSERM – Université Paris 7, Fontenay aux Roses,
France
2 Laboratoire de Radiosensibilité des Cellules Germinales, DRR / DSV/ CEA
– U566 INSERM – Université Paris 7, Fontenay aux Roses,
France
3 CNRS FRE 2358, Génétique Expérimentale et
Moléculaire, Orléans, France
* Author for correspondence (e-mail: pfouchet{at}armoise.saclay.cea.fr)
Accepted 13 October 2003
Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be
identified by the `Side Population' marker based on Hoechst 33342 efflux. We
show that mouse testicular cells also display a `Side Population' that express
Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in
hematopoietic cells. Inhibition of Hoechst efflux by specific BCRP1 inhibitor
Ko143 show that germinal `Side Population' phenotype is dependent on BCRP1
activity. Analysis of two well-defined models of altered spermatogenesis
(W/Wv mutants and cryptorchid male mice) and RNA
expression studies of differentiation markers demonstrate that germinal `Side
Population' contains spermatogonial cells. In addition,
6-integrin and Stra8 germinal stem cell markers, are
expressed in the `Side Population'. In vivo repopulation assay clearly
establishes that testis `Side Population' in adult mice is highly enriched in
male germ stem cells.
Key words: Spermatogenesis, Stem cells, BCRP1/ABCG2, Transplantation
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