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First published online 17 December 2003
doi: 10.1242/dev.00918

Development 131, 479-487 (2004)
Published by The Company of Biologists 2004
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`Side Population' cells in adult mouse testis express Bcrp1 gene and are enriched in spermatogonia and germinal stem cells

Bruno Lassalle1, Henri Bastos2, Jean Paul Louis3, Lydia Riou2, Jacques Testart1, Bernard Dutrillaux2, Pierre Fouchet2,* and Isabelle Allemand2

1 Laboratoire de Méiose et de Maturation Gamétique, DRR / DSV/ CEA – U566 INSERM – Université Paris 7, Fontenay aux Roses, France
2 Laboratoire de Radiosensibilité des Cellules Germinales, DRR / DSV/ CEA – U566 INSERM – Université Paris 7, Fontenay aux Roses, France
3 CNRS FRE 2358, Génétique Expérimentale et Moléculaire, Orléans, France

* Author for correspondence (e-mail: pfouchet{at}armoise.saclay.cea.fr)

Accepted 13 October 2003

Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be identified by the `Side Population' marker based on Hoechst 33342 efflux. We show that mouse testicular cells also display a `Side Population' that express Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in hematopoietic cells. Inhibition of Hoechst efflux by specific BCRP1 inhibitor Ko143 show that germinal `Side Population' phenotype is dependent on BCRP1 activity. Analysis of two well-defined models of altered spermatogenesis (W/Wv mutants and cryptorchid male mice) and RNA expression studies of differentiation markers demonstrate that germinal `Side Population' contains spermatogonial cells. In addition, {alpha}6-integrin and Stra8 germinal stem cell markers, are expressed in the `Side Population'. In vivo repopulation assay clearly establishes that testis `Side Population' in adult mice is highly enriched in male germ stem cells.

Key words: Spermatogenesis, Stem cells, BCRP1/ABCG2, Transplantation


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