spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 6 October 2004
doi: 10.1242/dev.01427

Development 131, 5405-5415 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01427v1
131/21/5405    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Devenport, D.
Right arrow Articles by Brown, N. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Devenport, D.
Right arrow Articles by Brown, N. H.

Morphogenesis in the absence of integrins: mutation of both Drosophila ß subunits prevents midgut migration

Danelle Devenport and Nicholas H. Brown*

Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Anatomy, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK

* Author for correspondence (e-mail: n.brown{at}gurdon.cam.ac.uk)

Accepted 1 September 2004

Two integrin ß subunits are encoded in the Drosophila genome. The ßPS subunit is widely expressed and heterodimers containing this subunit are required for many developmental processes. The second ß subunit, ß{nu}, is a divergent integrin expressed primarily in the midgut endoderm. To elucidate its function, we generated null mutations in the gene encoding ß{nu}. We find that ß{nu} is not required for viability or fertility, and overall the mutant flies are normal in appearance. However, we could observe ß{nu} function in the absence of ßPS. Consistent with its expression, removal of ß{nu} only enhanced the phenotype of ßPS in the developing midgut. In embryos lacking the zygotic contribution of ßPS, loss of ß{nu} resulted in enhanced separation between the midgut and the surrounding visceral mesoderm. In the absence of both maternal and zygotic ßPS, a delay in midgut migration was observed, but removing ß{nu} as well blocked migration completely. These results demonstrate that the second ß subunit can partially compensate for loss of ßPS integrins, and that integrins are essential for migration of the primordial midgut cells. The two ß subunits mediate midgut migration by distinct mechanisms: one that requires talin and one that does not. Other examples of developmental cell migration, such as that of the primordial germ cells, occurred normally in the absence of integrins. Having generated the tools to eliminate integrin function completely, we confirm that Drosophila integrins do not control proliferation as they do in mammals, and have identified {alpha}PS3 as a heterodimeric partner for ß{nu}.

Key words: Integrin, Migration, Drosophila, Extracellular matrix, Cell adhesion




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
A. M. O'Reilly, H.-H. Lee, and M. A. Simon
Integrins control the positioning and proliferation of follicle stem cells in the Drosophila ovary
J. Cell Biol., August 25, 2008; 182(4): 801 - 815.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
K. Jani and F. Schock
Zasp is required for the assembly of functional integrin adhesion sites
J. Cell Biol., December 31, 2007; 179(7): 1583 - 1597.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Z. Huang, U. Yazdani, K. L. Thompson-Peer, A. L. Kolodkin, and J. R. Terman
Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development
Development, June 15, 2007; 134(12): 2337 - 2347.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Murakami, D. Umetsu, Y. Maeyama, M. Sato, S. Yoshida, and T. Tabata
Focal adhesion kinase controls morphogenesis of the Drosophila optic stalk
Development, April 15, 2007; 134(8): 1539 - 1548.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Subramanian, B. Wayburn, T. Bunch, and T. Volk
Thrombospondin-mediated adhesion is essential for the formation of the myotendinous junction in Drosophila
Development, April 1, 2007; 134(7): 1269 - 1278.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
P. Bhandari, J. W. Gargano, M. M. Goddeeris, and M. S. Grotewiel
Behavioral Responses to Odorants in Drosophila Require Nervous System Expression of the {beta} Integrin Gene Myospheroid
Chem Senses, September 1, 2006; 31(7): 627 - 639.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. P. Levi, A. S. Ghabrial, and M. A. Krasnow
Drosophila talin and integrin genes are required for maintenance of tracheal terminal branches and luminal organization
Development, June 15, 2006; 133(12): 2383 - 2393.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. Ohkawa, J. O. Washburn, R. Sitapara, E. Sid, and L. E. Volkman
Specific Binding of Autographa californica M Nucleopolyhedrovirus Occlusion-Derived Virus to Midgut Cells of Heliothis virescens Larvae Is Mediated by Products of pif Genes Ac119 and Ac022 but Not by Ac115
J. Virol., December 15, 2005; 79(24): 15258 - 15264.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
H. Sano, A. D. Renault, and R. Lehmann
Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2
J. Cell Biol., November 21, 2005; 171(4): 675 - 683.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004