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First published online 7 January 2004
doi: 10.1242/dev.00955

Development 131, 643-656 (2004)
Published by The Company of Biologists 2004
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Drosophila ventral furrow morphogenesis: a proteomic analysis

Lei Gong*, Mamta Puri*, Mustafa Ünlü{dagger}, Margaret Young, Katherine Robertson, Surya Viswanathan, Arun Krishnaswamy, Susan R. Dowd and Jonathan S. Minden{ddagger}

Department of Biological Sciences and The NSF Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA

{ddagger} Author for correspondence (e-mail: minden{at}cmu.edu)

Accepted 30 October 2003

Ventral furrow formation is a key morphogenetic event during Drosophila gastrulation that leads to the internalization of mesodermal precursors. While genetic analysis has revealed the genes involved in the specification of ventral furrow cells, few of the structural proteins that act as mediators of ventral cell behavior have been identified. A comparative proteomics approach employing difference gel electrophoresis was used to identify more than fifty proteins with altered abundance levels or isoform changes in ventralized versus lateralized embryos. Curiously, the majority of protein differences between these embryos appeared well before gastrulation, only a few protein changes coincided with gastrulation, suggesting that the ventral cells are primed for cell shape change. Three proteasome subunits were found to differ between ventralized and lateralized embryos. RNAi knockdown of these proteasome subunits and time-dependent difference-proteins caused ventral furrow defects, validating the role of these proteins in ventral furrow morphogenesis.

Key words: Drosophila, Gastrulation, Ventral furrow formation, Proteomics




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