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First published online March 1, 2004
doi: 10.1242/10.1242/dev.01003

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polyhomeotic is required for somatic cell proliferation and differentiation during ovarian follicle formation in Drosophila

Karine Narbonne, Florence Besse, Jeanine Brissard-Zahraoui, Anne-Marie Pret^* and Denise Busson^*,

Institut Jacques Monod, (UMR 7592 - CNRS/Université Pierre et Marie Curie/Université Denis Diderot), Laboratoire de Génétique du Développement et Evolution, 2 place Jussieu, 75251 Paris Cedex 05, France

Author for correspondence (e-mail: bussond{at}ijm.jussieu.fr)

Accepted 25 November 2003

The polyhomeotic (ph) gene of Drosophila is a member of the Polycomb group (Pc-G) genes, which are required for maintenance of a repressed state of homeotic gene transcription, which stabilizes cell identity throughout development. The ph gene was recovered in the course of a gain-of-function screen aimed at identifying genes with a role during ovarian follicle formation in Drosophila, a process that involves coordinated proliferation and differentiation of two cell lineages, somatic and germline. Subsequent analysis revealed that ph loss-of-function mutations lead to production of follicles with greater or fewer than the normal number of germ cells associated with reduced proliferation of somatic prefollicular cells, abnormal prefollicular cell encapsulation of germline cysts and an excess of both interfollicular stalk cells and polar cells. Clonal analysis showed that ph function for follicle formation resides specifically in somatic cells and not in the germline. This is thus the first time that a role has been shown for a Pc-G gene during Drosophila folliculogenesis. In addition, we tested mutations in a number of other Pc-G genes, and two of them, Sex combs extra (Sce) and Sex comb on midleg (Scm), also displayed ovarian defects similar to those observed for ph. Our results provide a new model system, the Drosophila ovary, in which the function of Pc-G genes, distinct from that of control of homeotic gene expression, can be explored.

Key words: Oogenesis, polyhomeotic, Polycomb group, follicle cells, Drosophila

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