|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 3 March 2004
doi: 10.1242/dev.01044
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 The Carl C. Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai
School of Medicine, New York, NY 10029, USA
2 Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati,
OH 45229, USA
3 Department of Immunology, Medical Faculty/University Clinics, Ulm,
Germany
* Present address: Department of Public Health, Nara Medical University, Nara
634-8521, Japan
Present address: Paterson Institute for Cancer Research, Christie Hospital NHS
Trust, Wilmslow Road, Manchester M20 4BX, UK
Author for correspondence (e-mail:
gordon.keller{at}mssm.edu)
Accepted 3 December 2003
The cellular and molecular events regulating the induction and tissue-specific differentiation of endoderm are central to our understanding of the development and function of many organ systems. To define and characterize key components in this process, we have investigated the potential of embryonic stem (ES) cells to generate endoderm following their differentiation to embryoid bodies (EBs) in culture. We found that endoderm can be induced in EBs, either by limited exposure to serum or by culturing in the presence of activin A (activin) under serum-free conditions. By using an ES cell line with the green fluorescent protein (GFP) cDNA targeted to the brachyury locus, we demonstrate that endoderm develops from a brachyury+ population that also displays mesoderm potential. Transplantation of cells generated from activin-induced brachyury+ cells to the kidney capsule of recipient mice resulted in the development of endoderm-derived structures. These findings demonstrate that ES cells can generate endoderm in culture and, as such, establish this differentiation system as a unique murine model for studying the development and specification of this germ layer.
Key words: Stem cells, Endoderm
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Guo and M. Hebrok Stem Cells to Pancreatic {beta}-Cells: New Sources for Diabetes Cell Therapy Endocr. Rev., May 1, 2009; 30(3): 214 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ma, R. Latif, and T. F. Davies Thyrotropin-Independent Induction of Thyroid Endoderm from Embryonic Stem Cells by Activin A Endocrinology, April 1, 2009; 150(4): 1970 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Burtscher and H. Lickert Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo Development, March 15, 2009; 136(6): 1029 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Snykers, J. De Kock, V. Rogiers, and T. Vanhaecke In Vitro Differentiation of Embryonic and Adult Stem Cells into Hepatocytes: State of the Art Stem Cells, March 1, 2009; 27(3): 577 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Bernardo, C. H.-H. Cho, S. Mason, H. M. Docherty, R. A. Pedersen, L. Vallier, and K. Docherty Biphasic Induction of Pdx1 in Mouse and Human Embryonic Stem Cells Can Mimic Development of Pancreatic {beta}-Cells Stem Cells, February 1, 2009; 27(2): 341 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nakanishi, A. Kurisaki, Y. Hayashi, M. Warashina, S. Ishiura, M. Kusuda-Furue, and M. Asashima Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium FASEB J, January 1, 2009; 23(1): 114 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Zaret and M. Grompe Generation and Regeneration of Cells of the Liver and Pancreas Science, December 5, 2008; 322(5907): 1490 - 1494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Craft, D. M. Krisky, J. B. Wechuck, E. K. Lobenhofer, Y. Jiang, D. P. Wolfe, and J. C. Glorioso Herpes Simplex Virus-Mediated Expression of Pax3 and MyoD in Embryoid Bodies Results in Lineage-Related Alterations in Gene Expression Profiles Stem Cells, December 1, 2008; 26(12): 3119 - 3129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.S. Zaret, J. Watts, J. Xu, E. Wandzioch, S.T. Smale, and T. Sekiya Pioneer Factors, Genetic Competence, and Inductive Signaling: Programming Liver and Pancreas Progenitors from the Endoderm Cold Spring Harb Symp Quant Biol, November 26, 2008; (2008) sqb.2008.73.040v2. [Abstract] [PDF]
|
|
|
|
|
|
I. Drobinskaya, T. Linn, T. Saric, R. G. Bretzel, H. Bohlen, J. Hescheler, and E. Kolossov Scalable Selection of Hepatocyte- and Hepatocyte Precursor-Like Cells from Culture of Differentiating Transgenically Modified Murine Embryonic Stem Cells Stem Cells, September 1, 2008; 26(9): 2245 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sumi, N. Tsuneyoshi, N. Nakatsuji, and H. Suemori Defining early lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/{beta}-catenin, Activin/Nodal and BMP signaling Development, September 1, 2008; 135(17): 2969 - 2979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Rippon, S. Lane, M. Qin, N.-S. Ismail, M. R. Wilson, M. Takata, and A. E. Bishop Embryonic Stem Cells as a Source of Pulmonary Epithelium In Vitro and In Vivo Proceedings of the ATS, August 15, 2008; 5(6): 717 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Oliver-Krasinski and D. A. Stoffers On the origin of the {beta} cell Genes & Dev., August 1, 2008; 22(15): 1998 - 2021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ishii, K. Fukumitsu, K. Yasuchika, K. Adachi, E. Kawase, H. Suemori, N. Nakatsuji, I. Ikai, and S. Uemoto Effects of extracellular matrixes and growth factors on the hepatic differentiation of human embryonic stem cells Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G313 - G321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Narazaki, H. Uosaki, M. Teranishi, K. Okita, B. Kim, S. Matsuoka, S. Yamanaka, and J. K. Yamashita Directed and Systematic Differentiation of Cardiovascular Cells From Mouse Induced Pluripotent Stem Cells Circulation, July 29, 2008; 118(5): 498 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Shiraki, K. Umeda, N. Sakashita, M. Takeya, K. Kume, and S. Kume Differentiation of mouse and human embryonic stem cells into hepatic lineages. Genes Cells, July 1, 2008; 13(7): 731 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. McKinney-Freeman, C. Lengerke, I.-H. Jang, S. Schmitt, Y. Wang, M. Philitas, J. Shea, and G. Q. Daley Modulation of murine embryonic stem cell-derived CD41+c-kit+ hematopoietic progenitors by ectopic expression of Cdx genes Blood, May 15, 2008; 111(10): 4944 - 4953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Agarwal, K. L. Holton, and R. Lanza Efficient Differentiation of Functional Hepatocytes from Human Embryonic Stem Cells Stem Cells, May 1, 2008; 26(5): 1117 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Cano, I. C. Rulifson, P. W. Heiser, L. B. Swigart, S. Pelengaris, M. German, G. I. Evan, J. A. Bluestone, and M. Hebrok Regulated {beta}-Cell Regeneration in the Adult Mouse Pancreas Diabetes, April 1, 2008; 57(4): 958 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shiraki, T. Yoshida, K. Araki, A. Umezawa, Y. Higuchi, H. Goto, K. Kume, and S. Kume Guided Differentiation of Embryonic Stem Cells into Pdx1-Expressing Regional-Specific Definitive Endoderm Stem Cells, April 1, 2008; 26(4): 874 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Hay, D. Zhao, J. Fletcher, Z. A. Hewitt, D. McLean, A. Urruticoechea-Uriguen, J. R. Black, C. Elcombe, J. A. Ross, R. Wolf, et al. Efficient Differentiation of Hepatocytes from Human Embryonic Stem Cells Exhibiting Markers Recapitulating Liver Development In Vivo Stem Cells, April 1, 2008; 26(4): 894 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Thomas, S. Friedman, and R.-Y. Lin Thyroid stem cells: lessons from normal development and thyroid cancer Endocr. Relat. Cancer, March 1, 2008; 15(1): 51 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. K. Williamson, D. L. Smith, D. Blinco, R. D. Unwin, S. Pearson, C. Wilson, C. Miller, L. Lancashire, G. Lacaud, V. Kouskoff, et al. Quantitative Proteomics Analysis Demonstrates Post-transcriptional Regulation of Embryonic Stem Cell Differentiation to Hematopoiesis Mol. Cell. Proteomics, March 1, 2008; 7(3): 459 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Cho, N. Parashurama, E. Y. H. Park, K. Suganuma, Y. Nahmias, J. Park, A. W. Tilles, F. Berthiaume, and M. L. Yarmush Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure FASEB J, March 1, 2008; 22(3): 898 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Parashurama, Y. Nahmias, C. H. Cho, D. van Poll, A. W. Tilles, F. Berthiaume, and M. L. Yarmush Activin Alters the Kinetics of Endoderm Induction in Embryonic Stem Cells Cultured on Collagen Gels Stem Cells, February 1, 2008; 26(2): 474 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Serafimidis, I. Rakatzi, V. Episkopou, M. Gouti, and A. Gavalas Novel Effectors of Directed and Ngn3-Mediated Differentiation of Mouse Embryonic Stem Cells into Endocrine Pancreas Progenitors Stem Cells, January 1, 2008; 26(1): 3 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Fraker, S. Alvarez, P. Papadopoulos, J. Giraldo, W. Gu, C. Ricordi, L. Inverardi, and J. Dominguez-Bendala Enhanced Oxygenation Promotes -Cell Differentiation In Vitro Stem Cells, December 1, 2007; 25(12): 3155 - 3164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Duan, A. Catana, Y. Meng, N. Yamamoto, S. He, S. Gupta, S. S. Gambhir, and M. A. Zern Differentiation and Enrichment of Hepatocyte-Like Cells from Human Embryonic Stem Cells In Vitro and In Vivo Stem Cells, December 1, 2007; 25(12): 3058 - 3068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Jorgensen, J. Ahnfelt-Ronne, J. Hald, O. D. Madsen, P. Serup, and J. Hecksher-Sorensen An Illustrated Review of Early Pancreas Development in the Mouse Endocr. Rev., October 1, 2007; 28(6): 685 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jiang, M. Au, K. Lu, A. Eshpeter, G. Korbutt, G. Fisk, and A. S. Majumdar Generation of Insulin-Producing Islet-Like Clusters from Human Embryonic Stem Cells Stem Cells, August 1, 2007; 25(8): 1940 - 1953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Haremaki, S. T. Fraser, Y.-M. Kuo, M. H. Baron, and D. C. Weinstein Vertebrate Ctr1 coordinates morphogenesis and progenitor cell fate and regulates embryonic stem cell differentiation PNAS, July 17, 2007; 104(29): 12029 - 12034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Izumi, T. Era, H. Akimaru, M. Yasunaga, and S.-I. Nishikawa Dissecting the Molecular Hierarchy for Mesendoderm Differentiation Through a Combination of Embryonic Stem Cell Culture and RNA Interference Stem Cells, July 1, 2007; 25(7): 1664 - 1674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takenaga, M. Fukumoto, and Y. Hori Regulated Nodal signaling promotes differentiation of the definitive endoderm and mesoderm from ES cells J. Cell Sci., June 15, 2007; 120(12): 2078 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Vigneau, K. Polgar, G. Striker, J. Elliott, D. Hyink, O. Weber, H.-J. Fehling, G. Keller, C. Burrow, and P. Wilson Mouse Embryonic Stem Cell-Derived Embryoid Bodies Generate Progenitors That Integrate Long Term into Renal Proximal Tubules In Vivo J. Am. Soc. Nephrol., June 1, 2007; 18(6): 1709 - 1720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Karpowicz, T. Inoue, S. Runciman, B. Deveale, R. Seaberg, M. Gertsenstein, L. Byers, Y. Yamanaka, S. Tondat, J. Slevin, et al. Adhesion Is Prerequisite, But Alone Insufficient, to Elicit Stem Cell Pluripotency J. Neurosci., May 16, 2007; 27(20): 5437 - 5447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Mfopou, V. De Groote, X. Xu, H. Heimberg, and L. Bouwens Sonic Hedgehog and Other Soluble Factors from Differentiating Embryoid Bodies Inhibit Pancreas Development Stem Cells, May 1, 2007; 25(5): 1156 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Murtaugh Pancreas and beta-cell development: from the actual to the possible Development, February 1, 2007; 134(3): 427 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Torihashi, M. Kuwahara, T. Ogaeri, P. Zhu, M. Kurahashi, and T. Fujimoto Gut-Like Structures from Mouse Embryonic Stem Cells as an In Vitro Model for Gut Organogenesis Preserving Developmental Potential After Transplantation Stem Cells, December 1, 2006; 24(12): 2618 - 2626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gadue, T. L. Huber, P. J. Paddison, and G. M. Keller Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells PNAS, November 7, 2006; 103(45): 16806 - 16811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Lindsley, J. G. Gill, M. Kyba, T. L. Murphy, and K. M. Murphy Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm Development, October 1, 2006; 133(19): 3787 - 3796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yao, S. Chen, J. Clark, E. Hao, G. M. Beattie, A. Hayek, and S. Ding Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions PNAS, May 2, 2006; 103(18): 6907 - 6912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-G. Chen, Q. Wang, S.-L. Lin, C. D. Chang, J. Chung, and S.-Y. Ying Activin Signaling and Its Role in Regulation of Cell Proliferation, Apoptosis, and Carcinogenesis. Experimental Biology and Medicine, May 1, 2006; 231(5): 534 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Morrison and J. M. Brickman Conserved roles for Oct4 homologues in maintaining multipotency during early vertebrate development. Development, May 1, 2006; 133(10): 2011 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nishimura, T. S. Hamazaki, S. Komazaki, S. Kamimura, H. Okochi, and M. Asashima Ciliated Cells Differentiated from Mouse Embryonic Stem Cells Stem Cells, May 1, 2006; 24(5): 1381 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Rippon, J. M. Polak, M. Qin, and A. E. Bishop Derivation of Distal Lung Epithelial Progenitors from Murine Embryonic Stem Cells Using a Novel Three-Step Differentiation Protocol Stem Cells, May 1, 2006; 24(5): 1389 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Trounson The Production and Directed Differentiation of Human Embryonic Stem Cells Endocr. Rev., April 1, 2006; 27(2): 208 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Priddle, D. R. E. Jones, P. W. Burridge, and R. Patient Hematopoiesis from Human Embryonic Stem Cells: Overcoming the Immune Barrier in Stem Cell Therapies Stem Cells, April 1, 2006; 24(4): 815 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Forrai, K. Boyle, A. H. Hart, L. Hartley, S. Rakar, T. A. Willson, K. M. Simpson, A. W. Roberts, W. S. Alexander, A. K. Voss, et al. Absence of Suppressor of Cytokine Signalling 3 Reduces Self-Renewal and Promotes Differentiation in Murine Embryonic Stem Cells Stem Cells, March 1, 2006; 24(3): 604 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fujitani, S. Fujitani, D. F. Boyer, M. Gannon, Y. Kawaguchi, M. Ray, M. Shiota, R. W. Stein, M. A. Magnuson, and C. V.E. Wright Targeted deletion of a cis-regulatory region reveals differential gene dosage requirements for Pdx1 in foregut organ differentiation and pancreas formation Genes & Dev., January 15, 2006; 20(2): 253 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Amura, L. Marek, R. A. Winn, and L. E. Heasley Inhibited Neurogenesis in JNK1-Deficient Embryonic Stem Cells Mol. Cell. Biol., December 15, 2005; 25(24): 10791 - 10802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tada, T. Era, C. Furusawa, H. Sakurai, S. Nishikawa, M. Kinoshita, K. Nakao, T. Chiba, and S.-I. Nishikawa Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture Development, October 1, 2005; 132(19): 4363 - 4374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dravid, Z. Ye, H. Hammond, G. Chen, A. Pyle, P. Donovan, X. Yu, and L. Cheng Defining the Role of Wnt/{beta}-Catenin Signaling in the Survival, Proliferation, and Self-Renewal of Human Embryonic Stem Cells Stem Cells, October 1, 2005; 23(10): 1489 - 1501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Ng, R. P. Davis, L. Azzola, E. G. Stanley, and A. G. Elefanty Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation Blood, September 1, 2005; 106(5): 1601 - 1603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kubo, V. Chen, M. Kennedy, E. Zahradka, G. Q. Daley, and G. Keller The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation Blood, June 15, 2005; 105(12): 4590 - 4597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shiraki, C.-J. Lai, Y. Hishikari, and S. Kume TGF-{beta} signaling potentiates differentiation of embryonic stem cells to Pdx-1 expressing endodermal cells Genes Cells, June 1, 2005; 10(6): 503 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Keller Embryonic stem cell differentiation: emergence of a new era in biology and medicine Genes & Dev., May 15, 2005; 19(10): 1129 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. D'Souza, A. G. Elefanty, and G. Keller SCL/Tal-1 is essential for hematopoietic commitment of the hemangioblast but not for its development Blood, May 15, 2005; 105(10): 3862 - 3870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, L. Hou, F. Tang, W. Jiang, P. Wang, M. Ding, and H. Deng Inducing Embryonic Stem Cells to Differentiate into Pancreatic {beta} Cells by a Novel Three-Step Approach with Activin A and All-Trans Retinoic Acid Stem Cells, May 1, 2005; 23(5): 656 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Ng, L. Azzola, K. Sourris, L. Robb, E. G. Stanley, and A. G. Elefanty The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells Development, March 1, 2005; 132(5): 873 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Fair, B. A. Cairns, M. A. LaPaglia, M. Caballero, W. A. Pleasant, S. Hatada, H.-s. Kim, T. Gui, L. Pevny, A. A. Meyer, et al. Correction of factor IX deficiency in mice by embryonic stem cells differentiated in vitro PNAS, February 22, 2005; 102(8): 2958 - 2963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Stripp and S. D. Reynolds Bioengineered Lung Epithelium: Implications for Basic and Applied Studies in Lung Tissue Regeneration Am. J. Respir. Cell Mol. Biol., February 1, 2005; 32(2): 85 - 86. [Full Text] [PDF]
|
|
