spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 17 March 2004
doi: 10.1242/dev.01073

Development 131, 1729-1740 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01073v1
131/8/1729    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bucher, G.
Right arrow Articles by Klingler, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bucher, G.
Right arrow Articles by Klingler, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Divergent segmentation mechanism in the short germ insect Tribolium revealed by giant expression and function

Gregor Bucher and Martin Klingler*,{dagger}

Department for Biology II, Ludwig-Maximilian-University Munich, Luisenstraße 14, 80333 Munich, Germany

{dagger} Author for correspondence (e-mail: klingler{at}biologie.uni-erlangen.de)

Accepted 6 January 2004

Segmentation is well understood in Drosophila, where all segments are determined at the blastoderm stage. In the flour beetle Tribolium castaneum, as in most insects, the posterior segments are added at later stages from a posteriorly located growth zone, suggesting that formation of these segments may rely on a different mechanism. Nevertheless, the expression and function of many segmentation genes seem conserved between Tribolium and Drosophila. We have cloned the Tribolium ortholog of the abdominal gap gene giant. As in Drosophila, Tribolium giant is expressed in two primary domains, one each in the head and trunk. Although the position of the anterior domain is conserved, the posterior domain is located at least four segments anterior to that of Drosophila. Knockdown phenotypes generated with morpholino oligonucleotides, as well as embryonic and parental RNA interference, indicate that giant is required for segment formation and identity also in Tribolium. In giant-depleted embryos, the maxillary and labial segment primordia are normally formed but assume thoracic identity. The segmentation process is disrupted only in postgnathal metamers. Unlike Drosophila, segmentation defects are not restricted to a limited domain but extend to all thoracic and abdominal segments, many of which are specified long after giant expression has ceased. These data show that giant in Tribolium does not function as in Drosophila, and suggest that posterior gap genes underwent major regulatory and functional changes during the evolution from short to long germ embryogenesis.

Key words: giant, Short germ, Long germ, Segmentation, Tribolium, Gap gene, Abdomen, Krüppel, jaws, Drosophila, Evolution, Morpholino, Parental RNAi


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in Development:

Mind the gap

Development 2004 131: e802. [Full Text]  



This article has been cited by other articles:


Home page
 GeneticsHome page
L. R. Sanders, M. Patel, and J. W. Mahaffey
The Drosophila Gap Gene giant Has an Anterior Segment Identity Function Mediated Through disconnected and teashirt
Genetics, May 1, 2008; 179(1): 441 - 453.
[Abstract] [Full Text] [PDF]

Home page
 Phil Trans R Soc BHome page
A. D Peel
The evolution of developmental gene networks: lessons from comparative studies on holometabolous insects
Phil Trans R Soc B, April 27, 2008; 363(1496): 1539 - 1547.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. Marques-Souza, M. Aranda, and D. Tautz
Delimiting the conserved features of hunchback function for the trunk organization of insects
Development, March 1, 2008; 135(5): 881 - 888.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Konopova and M. Jindra
From the Cover: Juvenile hormone resistance gene Methoprene-tolerant controls entry into metamorphosis in the beetle Tribolium castaneum
PNAS, June 19, 2007; 104(25): 10488 - 10493.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. P. Choe, S. C. Miller, and S. J. Brown
A pair-rule gene circuit defines segments sequentially in the short-germ insect Tribolium castaneum
PNAS, April 25, 2006; 103(17): 6560 - 6564.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. C. Cerny, G. Bucher, R. Schroder, and M. Klingler
Breakdown of abdominal patterning in the Tribolium Kruppel mutant jaws
Development, December 15, 2005; 132(24): 5353 - 5363.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Mito, I. Sarashina, H. Zhang, A. Iwahashi, H. Okamoto, K. Miyawaki, Y. Shinmyo, H. Ohuchi, and S. Noji
Non-canonical functions of hunchback in segment patterning of the intermediate germ cricket Gryllus bimaculatus
Development, May 1, 2005; 132(9): 2069 - 2079.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. Z. Liu and T. C. Kaufman
Kruppel is a gap gene in the intermediate germband insect Oncopeltus fasciatus and is required for development of both blastoderm and germband-derived segments
Development, September 15, 2004; 131(18): 4567 - 4579.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004