|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online March 30, 2004
doi: 10.1242/10.1242/dev.01065
1 Department of Pediatrics, Graduate School of Medicine, Kyoto University, 54
Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
2 Department of Development and Differentiation, Institute for Frontier Medical
Science, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507,
Japan
3 Department of Medicine, Boston University School of Medicine, 88 East Newton
Street, Boston, MA 02118, USA
4 Research Center for Animal Life Science, Shiga University of Medical Science,
Tsukinowa-cho, Seta, Ohtsu, Shiga 520-2192, Japan
5 Division of Genetics, Institute of Medical Science, University of Tokyo, 4-6-1
Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan
* Author for correspondence (e-mail: tnakaha{at}kuhp.kyoto-u.ac.jp)
Accepted 8 January 2004
Although information about the development of primitive and definitive hematopoiesis has been elucidated in murine embryos and embryonic stem (ES) cells, there have been few in vitro studies of these processes in primates. In this study, we investigated hematopoietic differentiation from cynomolgus monkey ES cells grown on OP9, a stromal cell line deficient in macrophage colony-stimulating factor. Primitive erythrocytes (EryP) and definitive erythrocytes (EryD) developed sequentially from ES cells in the culture system; this was confirmed by immunostaining and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of embryonic, fetal and adult globin genes. EryP were detected on day 8 without exogenous erythropoietin (EPO), whereas EryD appeared on day 16 and had an indispensable requirement for exogenous EPO. RT-PCR analysis of the cultures revealed a sequential expression of genes associated with primitive and definitive hematopoietic development that was equivalent to that seen during primate ontogeny in vivo. Vascular endothelial growth factor (VEGF) increased, in a dose-dependent manner, not only the number of floating hematopoietic cells, but also the number of adherent hematopoietic cell clusters containing CD34-positive immature progenitors. In colony assays, exogenous VEGF also had a dose-dependent stimulatory effect on the generation of primitive erythroid colonies. More efficient primitive and definitive erythropoiesis was induced by re-plating sorted CD34-positive cells. Thus, this system reproduces early hematopoietic development in vitro and can serve as a model for analyzing the mechanisms of hematopoietic development in primates.
Key words: ES cells, Primate, Primitive hematopoiesis, Definitive hematopoiesis
This article has been cited by other articles:
|
|
 |
|
  F. Ma, N. Kambe, D. Wang, G. Shinoda, H. Fujino, K. Umeda, A. Fujisawa, L. Ma, H. Suemori, N. Nakatsuji, et al. Direct Development of Functionally Mature Tryptase/Chymase Double-Positive Connective Tissue-Type Mast Cells from Primate Embryonic Stem Cells Stem Cells, March 1, 2008; 26(3): 706 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Qiu, E. N. Olivier, M. Velho, and E. E. Bouhassira Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells Blood, February 15, 2008; 111(4): 2400 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Baba, T. Heike, M. Yoshimoto, K. Umeda, H. Doi, T. Iwasa, X. Lin, S. Matsuoka, M. Komeda, and T. Nakahata Flk1+ cardiac stem/progenitor cells derived from embryonic stem cells improve cardiac function in a dilated cardiomyopathy mouse model Cardiovasc Res, October 1, 2007; 76(1): 119 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Baba, T. Heike, K. Umeda, T. Iwasa, S. Kaichi, Y. Hiraumi, H. Doi, M. Yoshimoto, M. Kanatsu-Shinohara, T. Shinohara, et al. Generation of Cardiac and Endothelial Cells from Neonatal Mouse Testis-Derived Multipotent Germline Stem Cells Stem Cells, June 1, 2007; 25(6): 1375 - 1383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tsuchiya, T. Heike, S. Baba, H. Fujino, K. Umeda, Y. Matsuda, M. Nomoto, T. Ichida, Y. Aoyagi, and T. Nakahata Long-Term Culture of Postnatal Mouse Hepatic Stem/Progenitor Cells and Their Relative Developmental Hierarchy Stem Cells, April 1, 2007; 25(4): 895 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Shinoda, K. Umeda, T. Heike, M. Arai, A. Niwa, F. Ma, H. Suemori, H. Y. Luo, D. H. K. Chui, R. Torii, et al. {alpha}4-Integrin+ endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells Blood, March 15, 2007; 109(6): 2406 - 2415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rajesh, N. Chinnasamy, S. M. Mitalipov, D. P. Wolf, I. Slukvin, J. A. Thomson, and A. F. Shaaban Differential Requirements for Hematopoietic Commitment Between Human and Rhesus Embryonic Stem Cells Stem Cells, February 1, 2007; 25(2): 490 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Umeda, T. Heike, M. Nakata-Hizume, A. Niwa, M. Arai, G. Shinoda, F. Ma, H. Suemori, H. Y. Luo, D. H. K. Chui, et al. Sequential Analysis of {alpha}- and {beta}-Globin Gene Expression During Erythropoietic Differentiation from Primate Embryonic Stem Cells Stem Cells, December 1, 2006; 24(12): 2627 - 2636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kurita, E. Sasaki, T. Yokoo, T. Hiroyama, K. Takasugi, H. Imoto, K. Izawa, Y. Dong, T. Hashiguchi, Y. Soda, et al. Tal1/Scl Gene Transduction Using a Lentiviral Vector Stimulates Highly Efficient Hematopoietic Cell Differentiation from Common Marmoset (Callithrix jacchus) Embryonic Stem Cells Stem Cells, September 1, 2006; 24(9): 2014 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Umeda, T. Heike, M. Yoshimoto, G. Shinoda, M. Shiota, H. Suemori, H. Y. Luo, D. H. K. Chui, R. Torii, M. Shibuya, et al. Identification and Characterization of Hemoangiogenic Progenitors During Cynomolgus Monkey Embryonic Stem Cell Differentiation Stem Cells, May 1, 2006; 24(5): 1348 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Gale, A. Frolov, X. Han, P. E. Bickel, L. Cao, A. Bowcock, J. E. Schaffer, and D. S. Ory A Regulatory Role for 1-Acylglycerol-3-phosphate-O-acyltransferase 2 in Adipocyte Differentiation J. Biol. Chem., April 21, 2006; 281(16): 11082 - 11089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Priddle, D. R. E. Jones, P. W. Burridge, and R. Patient Hematopoiesis from Human Embryonic Stem Cells: Overcoming the Immune Barrier in Stem Cell Therapies Stem Cells, April 1, 2006; 24(4): 815 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. I. Slukvin, M. A. Vodyanik, J. A. Thomson, M. E. Gumenyuk, and K.-D. Choi Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway. J. Immunol., March 1, 2006; 176(5): 2924 - 2932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Olsen, D. L. Stachura, and M. J. Weiss Designer blood: creating hematopoietic lineages from embryonic stem cells Blood, February 15, 2006; 107(4): 1265 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sasaki, K. Hanazawa, R. Kurita, A. Akatsuka, T. Yoshizaki, H. Ishii, Y. Tanioka, Y. Ohnishi, H. Suemizu, A. Sugawara, et al. Establishment of Novel Embryonic Stem Cell Lines Derived from the Common Marmoset (Callithrix jacchus) Stem Cells, September 1, 2005; 23(9): 1304 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Vodyanik, J. A. Bork, J. A. Thomson, and I. I. Slukvin Human embryonic stem cell-derived CD34+ cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential Blood, January 15, 2005; 105(2): 617 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Carotta, S. Pilat, A. Mairhofer, U. Schmidt, H. Dolznig, P. Steinlein, and H. Beug Directed differentiation and mass cultivation of pure erythroid progenitors from mouse embryonic stem cells Blood, September 15, 2004; 104(6): 1873 - 1880. [Abstract] [Full Text] [PDF]
|
|
