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First published online 31 March 2004
doi: 10.1242/dev.01093
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1 Zebrafish Neurogenetics Junior Research Group, Institute of Virology,
Technical University-Munich, Trogerstrasse 4b, D-81675 Munich, Germany and
GSF-National Research Center for Environment and Health, Institute of
Developmental Genetics, Ingolstaedter Landstrasse 1, D-85764 Neuherberg,
Germany
2 Institut de Génétique et de Biologie Moléculaire et
Cellulaire, CNRS/INSERM/ULP, 1 rue Laurent Fries, BP 10142, F-67404 Illkirch
Cedex, C.U. de Strasbourg, France
Author for correspondence (e-mail:
bally{at}gsf.de)
Accepted 21 January 2004
Neurogenesis in both vertebrates and invertebrates is tightly controlled in time and space involving both positive and negative regulators. We report here that the bHLH factor Her5 acts as a prepattern gene to prevent neurogenesis in the anlage of the midbrain/hindbrain boundary in the zebrafish neural plate. This involves selective suppression of both neurogenin1 (ngn1) and coe2 mRNA expression in a process that is independent of Notch signalling, and where inhibition of either ngn1 or coe2 expression is sufficient to prevent neuronal differentiation across the midbrain-hindbrain boundary. A ngn1 transgene faithfully responds to Her5 and deletion analysis of the transgene identifies an E-box in a ngn1 upstream enhancer to be required for repression by Her5. Together our data demonstrate a role of Her5 as a prepattern factor in the spatial definition of proneural domains in the zebrafish neural plate, in a manner similar to its Drosophila homologue Hairy.
Key words: Her5, Hairy, Midbrain-hindbrain boundary, Zebrafish, Neurogenesis, Pre-patterning
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