spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 1 June 2005
doi: 10.1242/dev.01878


Development 132, 2991-3002 (2005)
Published by The Company of Biologists 2005


This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01878v1
132/13/2991    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kriks, S.
Right arrow Articles by Goulding, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kriks, S.
Right arrow Articles by Goulding, M.

Gsh2 is required for the repression of Ngn1 and specification of dorsal interneuron fate in the spinal cord

Sonja Kriks1, Guillermo M. Lanuza1,*, Rumiko Mizuguchi1,*, Masato Nakafuku2 and Martyn Goulding1,{dagger}

1 Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
2 Department of Cell and Developmental Biology, Children's Medical Research Foundation, 3333 Burnet Avenue, Cincinnati, OH 45229, USA

{dagger} Author for correspondence (e-mail: goulding{at}salk.edu)

Accepted 25 April 2005

The molecular programs that specify progenitors in the dorsal spinal cord remain poorly defined. The homeodomain transcription factor Gsh2 is expressed in the progenitors of three dorsal interneuron subtypes, dI3, dI4 and dI5 neurons, whereas Gsh1 is only expressed in dI4 and dI5 progenitors. Mice lacking Gsh2 exhibit a selective loss of dI3 interneurons that is accompanied by an expansion of the dI2 progenitor domain. In Gsh2 mutant embryos, expression of the proneural bHLH protein Mash1 is downregulated in dI3 neural progenitors, with Mash1 mutants exhibiting a concordant reduction in dI3 neurons. Conversely, overexpression of Gsh2 and Mash1 leads to the ectopic production of dI3 neurons and a concomitant repression of Ngn1 expression. Our results provide evidence that genetic interactions involving repression of Ngn1 by Gsh2 promote the differentiation of dI3 neurons from class A progenitors.

Key words: Spinal cord, Gsh2, Gsh1, Mash1, Ngn1, Interneurons




This article has been cited by other articles:


Home page
DevelopmentHome page
M. Sugimori, M. Nagao, N. Bertrand, C. M. Parras, F. Guillemot, and M. Nakafuku
Combinatorial actions of patterning and HLH transcription factors in the spatiotemporal control of neurogenesis and gliogenesis in the developing spinal cord
Development, April 15, 2007; 134(8): 1617 - 1629.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
G. Zhao, G. Boekhoff-Falk, B. A. Wilson, and J. B. Skeath
Linking pattern formation to cell-type specification: Dichaete and Ind directly repress achaete gene expression in the Drosophila CNS
PNAS, March 6, 2007; 104(10): 3847 - 3852.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
J. Battiste, A. W. Helms, E. J. Kim, T. K. Savage, D. C. Lagace, C. D. Mandyam, A. J. Eisch, G. Miyoshi, and J. E. Johnson
Ascl1 defines sequentially generated lineage-restricted neuronal and oligodendrocyte precursor cells in the spinal cord
Development, January 15, 2007; 134(2): 285 - 293.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
D. E. G. McNay, M. Pelling, S. Claxton, F. Guillemot, and S.-L. Ang
Mash1 Is Required for Generic and Subtype Differentiation of Hypothalamic Neuroendocrine Cells
Mol. Endocrinol., July 1, 2006; 20(7): 1623 - 1632.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
S. M. Glasgow, R. M. Henke, R. J. MacDonald, C. V. E. Wright, and J. E. Johnson
Ptf1a determines GABAergic over glutamatergic neuronal cell fate in the spinal cord dorsal horn
Development, December 15, 2005; 132(24): 5461 - 5469.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2005