|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online June 8, 2005
doi: 10.1242/10.1242/dev.01881
1 Masai Initiative Research Unit, RIKEN (The Institute of Physical and Chemical
Research), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
2 Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute
and CREST, JST, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
* Author for correspondence (e-mail: imasai{at}postman.riken.jp)
Accepted 27 April 2005
In the developing vertebrate retina, progenitor cells initially proliferate but begin to produce postmitotic neurons when neuronal differentiation occurs. However, the mechanism that determines whether retinal progenitor cells continue to proliferate or exit from the cell cycle and differentiate is largely unknown. Here, we report that histone deacetylase 1 (Hdac1) is required for the switch from proliferation to differentiation in the zebrafish retina. We isolated a zebrafish mutant, ascending and descending (add), in which retinal cells fail to differentiate into neurons and glial cells but instead continue to proliferate. The cloning of the add gene revealed that it encodes Hdac1. Furthermore, the ratio of the number of differentiating cells to that of proliferating cells increases in proportion to Hdac activity, suggesting that Hdac proteins regulate a crucial step of retinal neurogenesis in zebrafish. Canonical Wnt signaling promotes the proliferation of retinal cells in zebrafish, and Notch signaling inhibits neuronal differentiation through the activation of a neurogenic inhibitor, Hairy/Enhancer-of-split (Hes). We found that both the Wnt and Notch/Hes pathways are activated in the add mutant retina. The cell-cycle progression and the upregulation of Hes expression in the add mutant retina can be inhibited by the blockade of Wnt and Notch signaling, respectively. These data suggest that Hdac1 antagonizes these pathways to promote cell-cycle exit and the subsequent neurogenesis in zebrafish retina. Taken together, these data suggest that Hdac1 functions as a dual switch that suppresses both cell-cycle progression and inhibition of neurogenesis in the zebrafish retina.
Key words: Cell cycle, Danio rerio, Histone deacetylase, Neurogenesis, Notch, Retina, Wnt, Zebrafish
Related articles in Development:
This article has been cited by other articles:
|
|
 |
|
  M. Yamaguchi, N. Fujimori-Tonou, Y. Yoshimura, T. Kishi, H. Okamoto, and I. Masai Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53 Development, April 1, 2008; 135(7): 1247 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bessa, M. J. Tavares, J. Santos, H. Kikuta, M. Laplante, T. S. Becker, J. L. Gomez-Skarmeta, and F. Casares meis1 regulates cyclin D1 and c-myc expression, and controls the proliferation of the multipotent cells in the early developing zebrafish eye Development, March 1, 2008; 135(5): 799 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Heine, E. Dohle, K. Bumsted-O'Brien, D. Engelkamp, and D. Schulte Evidence for an evolutionary conserved role of homothorax/Meis1/2 during vertebrate retina development Development, March 1, 2008; 135(5): 805 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Baye and B. A. Link Interkinetic Nuclear Migration and the Selection of Neurogenic Cell Divisions during Vertebrate Retinogenesis J. Neurosci., September 19, 2007; 27(38): 10143 - 10152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-Y. Kim, E.-J. Ann, J.-Y. Kim, J.-S. Mo, J.-H. Park, S.-Y. Kim, M.-S. Seo, and H.-S. Park Tip60 Histone Acetyltransferase Acts as a Negative Regulator of Notch1 Signaling by Means of Acetylation Mol. Cell. Biol., September 15, 2007; 27(18): 6506 - 6519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhu, J. Zhang, J. Tollkuhn, R. Ohsawa, E. H. Bresnick, F. Guillemot, R. Kageyama, and M. G. Rosenfeld Sustained Notch signaling in progenitors is required for sequential emergence of distinct cell lineages during organogenesis Genes & Dev., October 1, 2006; 20(19): 2739 - 2753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Cho and C. L. Cepko Wnt2b/{beta}-catenin-mediated canonical Wnt signaling determines the peripheral fates of the chick eye Development, August 15, 2006; 133(16): 3167 - 3177. [Abstract] [Full Text] [PDF]
|
|
