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First published online 17 August 2005
doi: 10.1242/dev.01965

Development 132, 4075-4085 (2005)
Published by The Company of Biologists 2005
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Drosophila Myt1 is a Cdk1 inhibitory kinase that regulates multiple aspects of cell cycle behavior during gametogenesis

Zhigang Jin1, Ellen M. Homola1, Philip Goldbach2, YunHee Choi1, Julie A. Brill2 and Shelagh D. Campbell1,*

1 Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada
2 Program in Developmental Biology, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada

* Author for correspondence (e-mail: shelagh.campbell{at}ualberta.ca)

Accepted 27 June 2005

The metazoan Wee1-like kinases Wee1 and Myt1 regulate the essential mitotic regulator Cdk1 by inhibitory phosphorylation. This regulatory mechanism, which prevents Cdk1 from triggering premature mitotic events, is also induced during the DNA damage response and used to coordinate cell proliferation with crucial developmental events. Despite the previously demonstrated role for Myt1 regulation of Cdk1 during meiosis, relatively little is known of how Myt1 functions at other developmental stages. To address this issue, we have undertaken a functional analysis of Drosophila Myt1 that has revealed novel developmental roles for this conserved cell cycle regulator during gametogenesis. Notably, more proliferating cells were observed in myt1 mutant testes and ovaries than controls. This can partly be attributed to ectopic division of germline-associated somatic cells in myt1 mutants, suggesting that Myt1 serves a role in regulating exit from the cell cycle. Moreover, mitotic index measurements suggested that germline stem cells proliferate more rapidly, in myt1 mutant females. In addition, male myt1 germline cells occasionally undergo an extra mitotic division, resulting in meiotic cysts with twice the normal numbers of cells. Based on these observations, we propose that Myt1 serves unique Cdk1 regulatory functions required for efficient coupling of cell differentiation with cell cycle progression.

Key words: Myt1, Wee1, Cdk1, Cdc25, Meiosis, Mitosis, Spermatogenesis, Oogenesis




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[Abstract] [Full Text] [PDF]


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