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First published online 1 September 2005
doi: 10.1242/dev.02014

Development 132, 4327-4338 (2005)
Published by The Company of Biologists 2005
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Toutatis, a TIP5-related protein, positively regulates Pannier function during Drosophila neural development

Luc Vanolst, Catherine Fromental-Ramain and Philippe Ramain*

Institut de Génétique et de Biologie Moléculaire et Cellulaire, Department of Developmental Biology, CNRS/INSERM/ULP, Boite Postale 10142, 67404 Illkirch Cedex, France

* Author for correspondence (e-mail: phr{at}igbmc.u-strasbg.fr)

Accepted 28 July 2005

The GATA factor Pannier (Pnr) activates proneural expression through binding to a remote enhancer of the achaete-scute (ac-sc) complex. Chip associates both with Pnr and with the (Ac-Sc)-Daughterless heterodimer bound to the ac-sc promoters to give a proneural complex that facilitates enhancer-promoter communication during development. Using a yeast two-hybrid screening, we have identified Toutatis (Tou), which physically interacts with both Pnr and Chip. Loss-of-function and gain-of-function experiments indicate that Tou cooperates with Pnr and Chip during neural development. Tou shares functional domains with chromatin remodelling proteins, including TIP5 (termination factor TTFI-interacting protein 5) of NoRC (nucleolar remodelling complex), which mediates repression of RNA polymerase 1 transcription. In contrast, Tou acts positively to activate proneural gene expression. Moreover, we show that Iswi associates with Tou, Pnr and Chip, and is also required during Pnr-driven neural development. The results suggest that Tou and Iswi may belong to a complex that directly regulates the activity of Pnr and Chip during enhancer-promoter communication, possibly through chromatin remodelling.

Key words: Toutatis, Pnr, Chip, Iswi, Neural development, achaete-scute, Enhancer-promoter communication, Drosophila melanogaster


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