spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 1 September 2005
doi: 10.1242/dev.02005

Development 132, 4363-4374 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.02005v1
132/19/4363    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tada, S.
Right arrow Articles by Nishikawa, S.-I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tada, S.
Right arrow Articles by Nishikawa, S.-I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture

Shinsuke Tada1,2, Takumi Era1,*, Chikara Furusawa3, Hidetoshi Sakurai1, Satomi Nishikawa1, Masaki Kinoshita1, Kazuki Nakao4, Tsutomu Chiba2 and Shin-Ichi Nishikawa1

1 Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan
2 Department of Gastroenterology and Hepatology, Graduate school of Medicine, Kyoto University, 54 Shogoinkawara-cho, Sakyo-ku, Kyoto, 606-8507, Japan
3 Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 2-1 Yamada-oka, Suita City, Osaka, 565-0871, Japan
4 Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan

* Author for correspondence (e-mail: tera{at}cdb.riken.jp)

Accepted 19 July 2005

Bipotent mesendoderm that can give rise to both endoderm and mesoderm is an established entity from C. elegans to zebrafish. Although previous studies in mouse embryo indicated the presence of bi-potent mesendoderm cells in the organizer region, characterization of mesendoderm and its differentiation processes are still unclear. As bi-potent mesendoderm is implicated as the major precursor of definitive endoderm, its identification is also essential for exploring the differentiation of definitive endoderm. In this study, we have established embryonic stem (ES) cell lines that carry GFP gene in the goosecoid (Gsc) gene locus and have investigated the differentiation course of mesendodermal cells using Gsc expression as a marker. Our results show that mesendoderm is represented as a Gsc-GFP+E-cadherin(ECD)+PDGFR{alpha}({alpha}R)+ population and is selectively induced from ES cells under defined conditions containing either activin or nodal. Subsequently, it diverges to Gsc+ECD+{alpha}R- and Gsc+ECD-{alpha}R+ intermediates that eventually differentiate into definitive endoderm and mesodermal lineages, respectively. The presence of mesendodermal cells in nascent Gsc+ECD+{alpha}R+ population was also confirmed by single cell analysis. Finally, we show that the defined culture condition and surface markers developed in this study are applicable for obtaining pure mesendodermal cells and their immediate progenies from genetically unmanipulated ES cells.

Key words: ES cell, Mesendoderm, Goosecoid, Endoderm, Mouse


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in Development:

A culture of bipotency

Development 2005 132: e1905. [Full Text]  



This article has been cited by other articles:


Home page
 DevelopmentHome page
M. Kinoshita, T. Era, L. M. Jakt, and S.-I. Nishikawa
The novel protein kinase Vlk is essential for stromal function of mesenchymal cells
Development, June 15, 2009; 136(12): 2069 - 2079.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Ma, R. Latif, and T. F. Davies
Thyrotropin-Independent Induction of Thyroid Endoderm from Embryonic Stem Cells by Activin A
Endocrinology, April 1, 2009; 150(4): 1970 - 1975.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
S. M. Lim, L. Pereira, M. S. Wong, C. E. Hirst, B. E. Van Vranken, M. Pick, A. Trounson, A. G. Elefanty, and E. G. Stanley
Enforced Expression of Mixl1 During Mouse ES Cell Differentiation Suppresses Hematopoietic Mesoderm and Promotes Endoderm Formation
Stem Cells, February 1, 2009; 27(2): 363 - 374.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. S. Bernardo, C. H.-H. Cho, S. Mason, H. M. Docherty, R. A. Pedersen, L. Vallier, and K. Docherty
Biphasic Induction of Pdx1 in Mouse and Human Embryonic Stem Cells Can Mimic Development of Pancreatic {beta}-Cells
Stem Cells, February 1, 2009; 27(2): 341 - 351.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Harada, A. Ogai, T. Takahashi, M. Kitakaze, H. Matsubara, and H. Oh
Crossveinless-2 Controls Bone Morphogenetic Protein Signaling during Early Cardiomyocyte Differentiation in P19 Cells
J. Biol. Chem., September 26, 2008; 283(39): 26705 - 26713.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. C. Hay, J. Fletcher, C. Payne, J. D. Terrace, R. C. J. Gallagher, J. Snoeys, J. R. Black, D. Wojtacha, K. Samuel, Z. Hannoun, et al.
Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling
PNAS, August 26, 2008; 105(34): 12301 - 12306.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Ishii, K. Fukumitsu, K. Yasuchika, K. Adachi, E. Kawase, H. Suemori, N. Nakatsuji, I. Ikai, and S. Uemoto
Effects of extracellular matrixes and growth factors on the hepatic differentiation of human embryonic stem cells
Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G313 - G321.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
H. Sakurai, Y. Okawa, Y. Inami, N. Nishio, and K.-i. Isobe
Paraxial Mesodermal Progenitors Derived from Mouse Embryonic Stem Cells Contribute to Muscle Regeneration via Differentiation into Muscle Satellite Cells
Stem Cells, July 1, 2008; 26(7): 1865 - 1873.
[Abstract] [Full Text] [PDF]

Home page
 Phil Trans R Soc BHome page
H. Ema and H. Nakauchi
Bloodlines of haematopoietic stem cell research in Japan
Phil Trans R Soc B, June 27, 2008; 363(1500): 2089 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
T. J. Nelson, R. S. Faustino, A. Chiriac, R. Crespo-Diaz, A. Behfar, and A. Terzic
CXCR4+/FLK-1+ Biomarkers Select a Cardiopoietic Lineage from Embryonic Stem Cells
Stem Cells, June 1, 2008; 26(6): 1464 - 1473.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
S. Agarwal, K. L. Holton, and R. Lanza
Efficient Differentiation of Functional Hepatocytes from Human Embryonic Stem Cells
Stem Cells, May 1, 2008; 26(5): 1117 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
N. Shiraki, T. Yoshida, K. Araki, A. Umezawa, Y. Higuchi, H. Goto, K. Kume, and S. Kume
Guided Differentiation of Embryonic Stem Cells into Pdx1-Expressing Regional-Specific Definitive Endoderm
Stem Cells, April 1, 2008; 26(4): 874 - 885.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
D. C. Hay, D. Zhao, J. Fletcher, Z. A. Hewitt, D. McLean, A. Urruticoechea-Uriguen, J. R. Black, C. Elcombe, J. A. Ross, R. Wolf, et al.
Efficient Differentiation of Hepatocytes from Human Embryonic Stem Cells Exhibiting Markers Recapitulating Liver Development In Vivo
Stem Cells, April 1, 2008; 26(4): 894 - 902.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
C. H. Cho, N. Parashurama, E. Y. H. Park, K. Suganuma, Y. Nahmias, J. Park, A. W. Tilles, F. Berthiaume, and M. L. Yarmush
Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure
FASEB J, March 1, 2008; 22(3): 898 - 909.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. P. Davis, E. S. Ng, M. Costa, A. K. Mossman, K. Sourris, A. G. Elefanty, and E. G. Stanley
Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors
Blood, February 15, 2008; 111(4): 1876 - 1884.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
T. Era, N. Izumi, M. Hayashi, S. Tada, S. Nishikawa, and S.-I. Nishikawa
Multiple Mesoderm Subsets Give Rise to Endothelial Cells, Whereas Hematopoietic Cells Are Differentiated Only from a Restricted Subset in Embryonic Stem Cell Differentiation Culture
Stem Cells, February 1, 2008; 26(2): 401 - 411.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
N. Parashurama, Y. Nahmias, C. H. Cho, D. van Poll, A. W. Tilles, F. Berthiaume, and M. L. Yarmush
Activin Alters the Kinetics of Endoderm Induction in Embryonic Stem Cells Cultured on Collagen Gels
Stem Cells, February 1, 2008; 26(2): 474 - 484.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Shimoda, M. Kanai-Azuma, K. Hara, S. Miyazaki, Y. Kanai, M. Monden, and J.-i. Miyazaki
Sox17 plays a substantial role in late-stage differentiation of the extraembryonic endoderm in vitro
J. Cell Sci., November 1, 2007; 120(21): 3859 - 3869.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. Bakre, A. Hoi, J. C. Y. Mong, Y. Y. Koh, K. Y. Wong, and L. W. Stanton
Generation of Multipotential Mesendodermal Progenitors from Mouse Embryonic Stem Cells via Sustained Wnt Pathway Activation
J. Biol. Chem., October 26, 2007; 282(43): 31703 - 31712.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
F. Lavial, H. Acloque, F. Bertocchini, D. J. MacLeod, S. Boast, E. Bachelard, G. Montillet, S. Thenot, H. M. Sang, C. D. Stern, et al.
The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells
Development, October 1, 2007; 134(19): 3549 - 3563.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Kunath, M. K. Saba-El-Leil, M. Almousailleakh, J. Wray, S. Meloche, and A. Smith
FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment
Development, August 15, 2007; 134(16): 2895 - 2902.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
N. Izumi, T. Era, H. Akimaru, M. Yasunaga, and S.-I. Nishikawa
Dissecting the Molecular Hierarchy for Mesendoderm Differentiation Through a Combination of Embryonic Stem Cell Culture and RNA Interference
Stem Cells, July 1, 2007; 25(7): 1664 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Takenaga, M. Fukumoto, and Y. Hori
Regulated Nodal signaling promotes differentiation of the definitive endoderm and mesoderm from ES cells
J. Cell Sci., June 15, 2007; 120(12): 2078 - 2090.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
S. Baba, T. Heike, K. Umeda, T. Iwasa, S. Kaichi, Y. Hiraumi, H. Doi, M. Yoshimoto, M. Kanatsu-Shinohara, T. Shinohara, et al.
Generation of Cardiac and Endothelial Cells from Neonatal Mouse Testis-Derived Multipotent Germline Stem Cells
Stem Cells, June 1, 2007; 25(6): 1375 - 1383.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. K. Mfopou, V. De Groote, X. Xu, H. Heimberg, and L. Bouwens
Sonic Hedgehog and Other Soluble Factors from Differentiating Embryoid Bodies Inhibit Pancreas Development
Stem Cells, May 1, 2007; 25(5): 1156 - 1165.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Liu, M. Asakura, H. Inoue, T. Nakamura, M. Sano, Z. Niu, M. Chen, R. J. Schwartz, and M. D. Schneider
Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells
PNAS, March 6, 2007; 104(10): 3859 - 3864.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. Anjos-Afonso and D. Bonnet
Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment
Blood, February 1, 2007; 109(3): 1298 - 1306.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. P. L. Tam, P.-L. Khoo, S. L. Lewis, H. Bildsoe, N. Wong, T. E. Tsang, J. M. Gad, and L. Robb
Sequential allocation and global pattern of movement of the definitive endoderm in the mouse embryo during gastrulation
Development, January 15, 2007; 134(2): 251 - 260.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. B. McLean, K. A. D'Amour, K. L. Jones, M. Krishnamoorthy, M. J. Kulik, D. M. Reynolds, A. M. Sheppard, H. Liu, Y. Xu, E. E. Baetge, et al.
Activin A Efficiently Specifies Definitive Endoderm from Human Embryonic Stem Cells Only When Phosphatidylinositol 3-Kinase Signaling Is Suppressed
Stem Cells, January 1, 2007; 25(1): 29 - 38.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Kriz, N. Agren, C. K. Lindholm, S. Lenell, J. Saldeen, J. Mares, and M. Welsh
The SHB Adapter Protein Is Required for Normal Maturation of Mesoderm during in Vitro Differentiation of Embryonic Stem Cells
J. Biol. Chem., November 10, 2006; 281(45): 34484 - 34491.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Gadue, T. L. Huber, P. J. Paddison, and G. M. Keller
Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells
PNAS, November 7, 2006; 103(45): 16806 - 16811.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
L. Gruen and L. Grabel
Concise Review: Scientific and Ethical Roadblocks to Human Embryonic Stem Cell Therapy
Stem Cells, October 1, 2006; 24(10): 2162 - 2169.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. Mesnard, M. Guzman-Ayala, and D. B. Constam
Nodal specifies embryonic visceral endoderm and sustains pluripotent cells in the epiblast before overt axial patterning
Development, July 1, 2006; 133(13): 2497 - 2505.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Yao, S. Chen, J. Clark, E. Hao, G. M. Beattie, A. Hayek, and S. Ding
Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions
PNAS, May 2, 2006; 103(18): 6907 - 6912.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
H. J. Rippon, J. M. Polak, M. Qin, and A. E. Bishop
Derivation of Distal Lung Epithelial Progenitors from Murine Embryonic Stem Cells Using a Novel Three-Step Differentiation Protocol
Stem Cells, May 1, 2006; 24(5): 1389 - 1398.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Fujitani, S. Fujitani, D. F. Boyer, M. Gannon, Y. Kawaguchi, M. Ray, M. Shiota, R. W. Stein, M. A. Magnuson, and C. V.E. Wright
Targeted deletion of a cis-regulatory region reveals differential gene dosage requirements for Pdx1 in foregut organ differentiation and pancreas formation
Genes & Dev., January 15, 2006; 20(2): 253 - 266.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005