|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online September 28, 2005
doi: 10.1242/10.1242/dev.02019
1 Institut de Génétique et de Biologie Moléculaire et
Cellulaire (IGBMC), CNRS, INSERM, ULP, BP 10142-67404, Illkirch, C.U. de
Strasbourg, France
2 Institut Clinique de la Souris (ICS), BP 10142, CU de Strasbourg, 67404
Illkirch, France
3 Collège de France, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05,
France
4 Unité Expression Génétique et Maladies, CNRS URA 1644,
Département de Biologie du Développement, Institut Pasteur,
75724 Paris, France
* Authors for correspondence (e-mail: metzger{at}igbmc.u-strasbg.fr and chambon{at}igbmc.u-strasbg.fr)
Accepted 1 August 2005
Animal SWI2/SNF2 protein complexes containing either the brahma (BRM) or brahma-related gene 1 (BRG1) ATPase are involved in nucleosome remodelling and may control the accessibility of sequence-specific transcription factors to DNA. In vitro studies have indicated that BRM and BRG1 could regulate the expression of distinct sets of genes. However, as mice lacking BRM are viable and fertile, BRG1 might efficiently compensate for BRM loss. By contrast, as Brg1-null fibroblasts are viable but Brg1-null embryos die during the peri-implantation stage, BRG1 might exert cell-specific functions. To further investigate the in vivo role of BRG1, we selectively ablated Brg1 in keratinocytes of the forming mouse epidermis. We show that BRG1 is selectively required for epithelial-mesenchymal interactions in limb patterning, and during keratinocyte terminal differentiation, in which BRM can partially substitute for BRG1. By contrast, neither BRM nor BRG1 are essential for the proliferation and early differentiation of keratinocytes, which may require other ATP-dependent nucleosome-remodelling complexes. Finally, we demonstrate that cell-specific targeted somatic mutations can be created at various times during the development of mouse embryos cell-specifically expressing the tamoxifen-activatable Cre-ERT2 recombinase.
Key words: Targeted somatic mutagenesis, Cre-Lox, Epidermis, Limb, SNF2ß-BRG1
Related articles in Development:
This article has been cited by other articles:
|
|
 |
|
  S. Glaros, G. M. Cirrincione, A. Palanca, D. Metzger, and D. Reisman Targeted Knockout of BRG1 Potentiates Lung Cancer Development Cancer Res., May 15, 2008; 68(10): 3689 - 3696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ochi, S. Hans, and M. Westerfield Smarcd3 Regulates the Timing of Zebrafish Myogenesis Onset J. Biol. Chem., February 8, 2008; 283(6): 3529 - 3536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.-P. Charles, M. Guitard, C. Leyvraz, B. Breiden, M. Haftek, Z. Haftek-Terreau, J.-C. Stehle, K. Sandhoff, and E. Hummler Postnatal Requirement of the Epithelial Sodium Channel for Maintenance of Epidermal Barrier Function J. Biol. Chem., February 1, 2008; 283(5): 2622 - 2630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Griffin, J. Brennan, and T. Magnuson The chromatin-remodeling enzyme BRG1 plays an essential role in primitive erythropoiesis and vascular development Development, February 1, 2008; 135(3): 493 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kashiwagi, B. A. Morgan, and K. Georgopoulos The chromatin remodeler Mi-2{beta} is required for establishment of the basal epidermis and normal differentiation of its progeny Development, April 15, 2007; 134(8): 1571 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Kwon, K.-i. Hibara, J. Pfluger, S. Bezhani, H. Metha, M. Aida, M. Tasaka, and D. Wagner A role for chromatin remodeling in regulation of CUC gene expression in the Arabidopsis cotyledon boundary Development, August 15, 2006; 133(16): 3223 - 3230. [Abstract] [Full Text] [PDF]
|
|
