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First published online September 28, 2005
doi: 10.1242/10.1242/dev.02043

Development 132, 4575-4586 (2005)
Published by The Company of Biologists 2005
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The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo

Clara Collart1,2, Kristin Verschueren1, Amer Rana2, James C. Smith2,* and Danny Huylebroeck1,*

1 Department of Developmental Biology (VIB-07), Flanders Interuniversity Institute for Biotechnology (VIB), and Laboratory of Molecular Biology (Celgen), University of Leuven, B-3000 Leuven, Belgium
2 Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK

* Authors for correspondence (e-mail: jim{at}gurdon.cam.ac.uk and danny.huylebroeck{at}med.kuleuven.be)

Accepted 10 August 2005

In this paper, we investigate the function of Smicl, a zinc-finger Smad-interacting protein that is expressed maternally in the Xenopus embryo. Inhibition of Smicl function by means of antisense morpholino oligonucleotides causes the specific downregulation of Chordin, a dorsally expressed gene encoding a secreted BMP inhibitor that is involved in mesodermal patterning and neural induction. Chordin is activated by Nodal-related signalling in an indirect manner, and we show here that Smicl is involved in a two-step process that is necessary for this activation. In the first, Smad3 (but not Smad2) activates expression of Xlim1 in a direct fashion. In the second, a complex containing Smicl and the newly induced Xlim1 induces expression of Chordin. As well as revealing the function of Smicl in the early embryo, our work yields important new insight in the regulation of Chordin and identifies functional differences between the activities of Smad2 and Smad3 in the Xenopus embryo.

Key words: Smicl, Xenopus, Chordin, Smad, Nodal, Spemann's organiser




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