|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online November 10, 2005
doi: 10.1242/10.1242/dev.02138
1 Section in Cell and Developmental Biology, Division of Biology, University of
California, San Diego, La Jolla, CA 92093, USA
2 Howard Hughes Medical Institute, Laboratory of Genetics, University of
Wisconsin-Madison, Madison, WI 53706, USA
* Author for correspondence (e-mail: mcginnis{at}biomail.ucsd.edu)
Accepted 28 September 2005
While testing the functions of deletion mutants in the Hox protein Ultrabithorax (Ubx), we found that the embryonic repression function of Ubx on Distal-less transcription in limb primordia is highly concentration dependent. The steep sigmoidal relationship between in vivo Ubx concentration and Distal-less repression is dependent on the Ubx YPWM motif. This suggests that Ubx cooperatively assembles a multi-protein repression complex on Distal-less regulatory DNA with the YPWM motif as a key protein-protein interface in this complex. Our deletion mutants also provide evidence for a transcriptional activation domain in the N-terminal 19 amino acids of Ubx. This proposed activation domain contains a variant of the SSYF motif that is found at the N termini of many Hox proteins, and is conserved in the activation domain of another Hox protein, Sex combs reduced. These results suggest that the N-terminal region containing the SSYF motif has been conserved in many Hox proteins for its role in transcriptional activation.
Key words: Ultrabithorax, Drosophila, Hox, Transcriptional activation, Transcriptional repression, Sex combs reduced
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in Development:
This article has been cited by other articles:
|
|
 |
|
  J. Jia, M. Maccarana, X. Zhang, M. Bespalov, U. Lindahl, and J.-P. Li Lack ofL-Iduronic Acid in Heparan Sulfate Affects Interaction with Growth Factors and Cell Signaling J. Biol. Chem., June 5, 2009; 284(23): 15942 - 15950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sivanantharajah and A. Percival-Smith Analysis of the Sequence and Phenotype of Drosophila Sex combs reduced Alleles Reveals Potential Functions of Conserved Protein Motifs of the Sex combs reduced Protein Genetics, May 1, 2009; 182(1): 191 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Garaulet, D. Foronda, M. Calleja, and E. Sanchez-Herrero Polycomb-dependent Ultrabithorax Hox gene silencing induced by high Ultrabithorax levels in Drosophila Development, October 1, 2008; 135(19): 3219 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, K. S. Matthews, and S. E. Bondos Multiple Intrinsically Disordered Sequences Alter DNA Binding by the Homeodomain of the Drosophila Hox Protein Ultrabithorax J. Biol. Chem., July 25, 2008; 283(30): 20874 - 20887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R Copley The animal in the genome: comparative genomics and evolution Phil Trans R Soc B, April 27, 2008; 363(1496): 1453 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Merabet, M. Saadaoui, N. Sambrani, B. Hudry, J. Pradel, M. Affolter, and Y. Graba A unique Extradenticle recruitment mode in the Drosophila Hox protein Ultrabithorax PNAS, October 23, 2007; 104(43): 16946 - 16951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Knosp, C. Saneyoshi, S. Shou, H. P. Bachinger, and H. S. Stadler Elucidation, Quantitative Refinement, and in Vivo Utilization of the HOXA13 DNA Binding Site J. Biol. Chem., March 2, 2007; 282(9): 6843 - 6853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shimizu, Y.-K. Bae, and M. Hibi Cdx-Hox code controls competence for responding to Fgfs and retinoic acid in zebrafish neural tissue Development, December 1, 2006; 133(23): 4709 - 4719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kobrossy, M. Rastegar, and M. Featherstone Interplay between Chromatin and Trans-acting Factors Regulating the Hoxd4 Promoter during Neural Differentiation J. Biol. Chem., September 8, 2006; 281(36): 25926 - 25939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. D. Shippy, C. D. Rogers, R. W. Beeman, S. J. Brown, and R. E. Denell The Tribolium castaneum Ortholog of Sex combs reduced Controls Dorsal Ridge Development Genetics, September 1, 2006; 174(1): 297 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Hittinger, D. L. Stern, and S. B. Carroll Pleiotropic functions of a conserved insect-specific Hox peptide motif Development, December 1, 2005; 132(23): 5261 - 5270. [Abstract] [Full Text] [PDF]
|
|
