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First published online 9 November 2005
doi: 10.1242/dev.02151
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1 Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, MO
64110, USA
2 Department of Anatomy and Cell Biology, University of Kansas School of
Medicine, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
* Author for correspondence (e-mail: tgx{at}stowers-institute.org)
Accepted 5 October 2005
In the Drosophila ovary, germline stem cell (GSC) self-renewal is
controlled by both extrinsic and intrinsic factors. The Bmp signal from niche
cells controls GSC self-renewal by directly repressing a Bam-dependent
differentiation pathway in GSCs. pelota (pelo), which has
been previously shown to be required for Drosophila male meiosis, was
identified in our genetic screen as a dominant suppressor of the dpp
overexpression-induced GSC tumor phenotype. In this study, we reveal the
unexpected new role of Pelo in controlling GSC self-renewal by repressing a
Bam-independent differentiation pathway. In pelo mutant ovaries, GSCs
are lost rapidly owing to differentiation. Results from genetic mosaic
analysis and germ cell-specific rescue show that it functions as an intrinsic
factor to control GSC self-renewal. In pelo mutant GSCs, Bmp
signaling activity detected by Dad-lacZ expression is downregulated,
but bam expression is still repressed. Furthermore, bam
mutant germ cells are still able to differentiate into cystocytes without
pelo function, indicating that Pelo is involved in repressing a
Bam-independent differentiation pathway. Consistent with its homology to the
eukaryotic translation release factor 1
, we show that Pelo is localized
to the cytoplasm of the GSC. Therefore, Pelo controls GSC self-renewal by
repressing a Bam-independent differentiation pathway possibly through
regulating translation. As Pelo is highly conserved from Drosophila
to mammals, it may also be involved in the regulation of adult stem cell
self-renewal in mammals, including humans.
Key words: Germline stem cells, Self-renewal, Pelota, Bmp, Differentiation, Drosophila
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