|
|
|
|||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 16 November 2005
doi: 10.1242/dev.02139
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||


1 Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Kanagawa
230-0045, Japan
2 Department of Botany, Graduate School of Science, Kyoto University,
Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
3 Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba
292-0818, Japan
Author for correspondence (e-mail:
twada{at}psc.riken.jp)
Accepted 29 September 2005
CAPRICE (CPC), a small, R3-type Myb-like protein, is a positive regulator of root hair development in Arabidopsis. Cell-to-cell movement of CPC is important for the differentiation of epidermal cells into trichoblasts (root hair cells). CPC is transported from atrichoblasts (hairless cells), where it is expressed, to trichoblasts, and generally accumulates in their nuclei. Using truncated versions of CPC fused to GFP, we identified a signal domain that is necessary and sufficient for CPC cell-to-cell movement. This domain includes the N-terminal region and a part of the Myb domain. Amino acid substitution experiments indicated that W76 and M78 in the Myb domain are critical for targeted transport, and that W76 is crucial for the nuclear accumulation of CPC:GFP. To evaluate the tissue-specificity of CPC movement, CPC:GFP was expressed in the stele using the SHR promoter and in trichoblasts using the EGL3 promoter. CPC:GFP was able to move from trichoblasts to atrichoblasts but could not exit from the stele, suggesting the involvement of tissue-specific regulatory factors in the intercellular movement of CPC. Analyses with a secretion inhibitor, Brefeldin A, and with an rhd3 mutant defective in the secretion process in root epidermis suggested that intercellular CPC movement is mediated through plasmodesmata. Furthermore, the fusion of CPC to tandem-GFPs defined the capability of CPC to increase the size exclusion limit of plasmodesmata.
Key words: Arabidopsis, Epidermis, CAPRICE, Myb, Protein movement
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati
Twitter What's this?
This article has been cited by other articles:
![]() |
J. Schiefelbein, S.-H. Kwak, Y. Wieckowski, C. Barron, and A. Bruex The gene regulatory network for root epidermal cell-type pattern formation in Arabidopsis J. Exp. Bot., April 1, 2009; 60(5): 1515 - 1521. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. H. Kang, V. Kirik, M. Hulskamp, K. H. Nam, K. Hagely, M. M. Lee, and J. Schiefelbein The MYB23 Gene Provides a Positive Feedback Loop for Cell Fate Specification in the Arabidopsis Root Epidermis PLANT CELL, April 1, 2009; 21(4): 1080 - 1094. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Simpson, C. Thomas, K. Findlay, E. Bayer, and A. J. Maule An Arabidopsis GPI-Anchor Plasmodesmal Neck Protein with Callose Binding Activity and Potential to Regulate Cell-to-Cell Trafficking PLANT CELL, February 1, 2009; 21(2): 581 - 594. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Tominaga, M. Iwata, R. Sano, K. Inoue, K. Okada, and T. Wada Arabidopsis CAPRICE-LIKE MYB 3 (CPL3) controls endoreduplication and flowering development in addition to trichome and root hair formation Development, April 1, 2008; 135(7): 1335 - 1345. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Wang, S.-H. Kwak, Q. Zeng, B. E. Ellis, X.-Y. Chen, J. Schiefelbein, and J.-G. Chen TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis Development, November 1, 2007; 134(21): 3873 - 3882. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Morohashi, M. Zhao, M. Yang, B. Read, A. Lloyd, R. Lamb, and E. Grotewold Participation of the Arabidopsis bHLH Factor GL3 in Trichome Initiation Regulatory Events Plant Physiology, November 1, 2007; 145(3): 736 - 746. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Muto, M. K. Watahiki, D. Nakamoto, M. Kinjo, and K. T. Yamamoto Specificity and Similarity of Functions of the Aux/IAA Genes in Auxin Signaling of Arabidopsis Revealed by Promoter-Exchange Experiments among MSG2/IAA19, AXR2/IAA7, and SLR/IAA14 Plant Physiology, May 1, 2007; 144(1): 187 - 196. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Cui, M. P. Levesque, T. Vernoux, J. W. Jung, A. J. Paquette, K. L. Gallagher, J. Y. Wang, I. Blilou, B. Scheres, and P. N. Benfey An Evolutionarily Conserved Mechanism Delimiting SHR Movement Defines a Single Layer of Endodermis in Plants Science, April 20, 2007; 316(5823): 421 - 425. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. P. Alvarez, I. Pekker, A. Goldshmidt, E. Blum, Z. Amsellem, and Y. Eshed Endogenous and Synthetic MicroRNAs Stimulate Simultaneous, Efficient, and Localized Regulation of Multiple Targets in Diverse Species PLANT CELL, May 1, 2006; 18(5): 1134 - 1151. [Abstract] [Full Text] [PDF] |
||||