|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 12 January 2005
doi: 10.1242/dev.01606
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, Singapore
138673
2 Centre for Biomedical Genetics, Department of Biomedical Science, University
of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
3 Department of Biological Sciences, 14 Science Drive 4, National University of
Singapore, Singapore 117543
Author for correspondence (e-mail:
sudipto{at}imcb.a-star.edu.sg)
Accepted 2 December 2004
Orthologues of nearly all of the core components of the Hedgehog signalling pathway, defined originally through genetic analysis in Drosophila, have now been discovered in vertebrates and shown to have highly conserved functions. The one striking exception to this rule is the kinesin-like protein Costal2, which plays a central role in controlling the activity of the zinc-finger-containing transcriptional regulator, Cubitus interruptus that modulates all Hedgehog-dependent target gene expression, but whose involvement in Hedgehog signalling has not been demonstrated in vertebrates. We report the cloning of a kinesin-related gene from the zebrafish that in structure as well as function, appears to represent the first vertebrate orthologue of costal2. Using a combination of genetic and biochemical analysis, we provide evidence that as in Drosophila, zebrafish Costal2 acts principally as an intracellular repressor of signal transduction, in conjunction with Suppressor of Fused, another protein that negatively regulates signalling in Hedgehog-responsive cells.
Key words: Hedgehog, Costal2, Suppressor of Fused, Zebrafish, Muscle
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in Development:
This article has been cited by other articles:
|
|
 |
|
  H. O.-L. Cheung, X. Zhang, A. Ribeiro, R. Mo, S. Makino, V. Puviindran, K. K. L. Law, J. Briscoe, and C.-c. Hui The Kinesin Protein Kif7 Is a Critical Regulator of Gli Transcription Factors in Mammalian Hedgehog Signaling Sci. Signal., June 23, 2009; 2(76): ra29 - ra29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Philipp, G. B. Fralish, A. R. Meloni, W. Chen, A. W. MacInnes, L. S. Barak, and M. G. Caron Smoothened Signaling in Vertebrates Is Facilitated by a G Protein-coupled Receptor Kinase Mol. Biol. Cell, December 1, 2008; 19(12): 5478 - 5489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Dessaud, A. P. McMahon, and J. Briscoe Pattern formation in the vertebrate neural tube: a sonic hedgehog morphogen-regulated transcriptional network Development, August 1, 2008; 135(15): 2489 - 2503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu, B. P. Srinivas, S. Y. Tay, A. Mak, X. Yu, S. G. P. Lee, H. Yang, K. R. Govindarajan, B. Leong, G. Bourque, et al. Genomewide Expression Profiling in the Zebrafish Embryo Identifies Target Genes Regulated by Hedgehog Signaling During Vertebrate Development Genetics, October 1, 2006; 174(2): 735 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Huangfu and K. V. Anderson Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates Development, January 1, 2006; 133(1): 3 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Chen, N. Gao, T. Kawakami, and P.-T. Chuang Mice Deficient in the Fused Homolog Do Not Exhibit Phenotypes Indicative of Perturbed Hedgehog Signaling during Embryonic Development Mol. Cell. Biol., August 15, 2005; 25(16): 7042 - 7053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Merchant, M. Evangelista, S.-M. Luoh, G. D. Frantz, S. Chalasani, R. A. D. Carano, M. van Hoy, J. Ramirez, A. K. Ogasawara, L. M. McFarland, et al. Loss of the Serine/Threonine Kinase Fused Results in Postnatal Growth Defects and Lethality Due to Progressive Hydrocephalus Mol. Cell. Biol., August 15, 2005; 25(16): 7054 - 7068. [Abstract] [Full Text] [PDF]
|
|
