|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 12 January 2005
doi: 10.1242/dev.01609
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Laboratory of Mammary Gland Biology and Tumorigenesis, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
e-mail: gs4d{at}nih.gov
Accepted 2 December 2004
It has been postulated that the stem cells of somatic tissues protect themselves from mutation and cancer risk by selective segregation of their template DNA strands. Self-renewing mammary epithelial stem cells that were originated during allometric growth of the mammary ducts in pubertal females were labeled using [3H]-thymidine (3HTdR). After a prolonged chase during which much of the branching duct morphogenesis was completed, 3HTdR-label retaining epithelial cells (LREC) were detected among the epithelium of the maturing glands. Labeling newly synthesized DNA in these glands with a different marker, 5-bromodeoxyuridine (5BrdU), resulted in the appearance of doubly labeled nuclei in a large percentage of the LREC. By contrast, label-retaining cells within the stroma did not incorporate 5BrdU during the pulse, indicating that they were not traversing the cell cycle. Upon chase, the second label (5BrdU) was distributed from the double-labeled LREC to unlabeled mammary cells while 3HTdR was retained. These results demonstrate that mammary LREC selectively retain their 3HTdR-labeled template DNA strands and pass newly synthesized 5BrdU-labeled DNA to their progeny during asymmetric divisions. Similar results were obtained in mammary transplants containing self-renewing, lacZ-positive epithelial cells suggesting that cells capable of expansive self-renewal may repopulate new mammary stem cell niches during the allometric growth of new mammary ducts.
Key words: Mammary, Stem cell, Asymmetric division, Autoradiography
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. V. Capuco, C. M. Evock-Clover, A. Minuti, and D. L. Wood In Vivo Expansion of the Mammary Stem/ Progenitor Cell Population by Xanthosine Infusion Experimental Biology and Medicine, April 1, 2009; 234(4): 475 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Wang, Y. Yin, H. Yuan, T. Sakamaki, H. Okano, and R. I. Glazer Musashi1 Modulates Mammary Progenitor Cell Expansion through Proliferin-Mediated Activation of the Wnt and Notch Pathways Mol. Cell. Biol., June 1, 2008; 28(11): 3589 - 3599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, M. T. Lewis, J. Huang, C. Gutierrez, C. K. Osborne, M.-F. Wu, S. G. Hilsenbeck, A. Pavlick, X. Zhang, G. C. Chamness, et al. Intrinsic Resistance of Tumorigenic Breast Cancer Cells to Chemotherapy J Natl Cancer Inst, May 7, 2008; 100(9): 672 - 679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Britt, A. Ashworth, and M. Smalley Pregnancy and the risk of breast cancer Endocr. Relat. Cancer, December 1, 2007; 14(4): 907 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Capuco Identification of Putative Bovine Mammary Epithelial Stem Cells by Their Retention of Labeled DNA Strands Experimental Biology and Medicine, November 1, 2007; 232(10): 1381 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Sleeman, H. Kendrick, D. Robertson, C. M. Isacke, A. Ashworth, and M. J. Smalley Dissociation of estrogen receptor expression and in vivo stem cell activity in the mammary gland J. Cell Biol., January 1, 2007; 176(1): 19 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cairns Cancer and the Immortal Strand Hypothesis Genetics, November 1, 2006; 174(3): 1069 - 1072. [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Visvader and G. J. Lindeman Mammary stem cells and mammopoiesis. Cancer Res., October 15, 2006; 66(20): 9798 - 9801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Armakolas and A. J. S. Klar Cell type regulates selective segregation of mouse chromosome 7 DNA strands in mitosis. Science, February 24, 2006; 311(5764): 1146 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kanatsu-Shinohara, N. Ogonuki, T. Iwano, J. Lee, Y. Kazuki, K. Inoue, H. Miki, M. Takehashi, S. Toyokuni, Y. Shinkai, et al. Genetic and epigenetic properties of mouse male germline stem cells during long-term culture Development, September 15, 2005; 132(18): 4155 - 4163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Woodward, M. S. Chen, F. Behbod, and J. M. Rosen On mammary stem cells J. Cell Sci., August 15, 2005; 118(16): 3585 - 3594. [Abstract] [Full Text] [PDF]
|
|
