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First published online 19 January 2005
doi: 10.1242/dev.01614
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1 Department of Biological Sciences, Stanford University, Stanford, CA 94305,
USA
2 Institute of Molecular and Cellular Biosciences, The University of Tokyo,
Tokyo 113-0032, Japan
* Author for correspondence (e-mail: lluo{at}stanford.edu)
Accepted 9 December 2004
Neural circuits are often remodeled after initial connections are
established. The mechanisms by which remodeling occurs, in particular whether
and how synaptically connected neurons coordinate their reorganization, are
poorly understood. In Drosophila, olfactory projection neurons (PNs)
receive input by synapsing with olfactory receptor neurons in the antennal
lobe and relay information to the mushroom body (MB) calyx and lateral horn.
Here we show that embryonic-born PNs participate in both the larval and adult
olfactory circuits. In the larva, these neurons generally innervate a single
glomerulus in the antennal lobe and one or two glomerulus-like substructures
in the MB calyx. They persist in the adult olfactory circuit and are
prespecified by birth order to innervate a subset of glomeruli distinct from
larval-born PNs. Developmental studies indicate that these neurons undergo
stereotyped pruning of their dendrites and axon terminal branches locally
during early metamorphosis. Electron microscopy analysis reveals that these
PNs synapse with MB 
neurons in the larval calyx and that these
synaptic profiles are engulfed by glia during early metamorphosis. As with MB
neurons, PN pruning requires cell-autonomous reception of the nuclear
hormone ecdysone. Thus, these synaptic partners are independently programmed
to prune their dendrites and axons.
Key words: Olfaction, Projection neuron, Metamorphosis, Pruning, Steroid hormone, TGFß signaling, Antennal lobe, Mushroom body, Lateral horn, Drosophila
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