QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 2 February 2005
doi: 10.1242/dev.01664

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: dev.01664v1 132/5/1047    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Leight, E. R. Articles by Kornfeld, K. Search for Related Content PubMed PubMed Citation Articles by Leight, E. R. Articles by Kornfeld, K.

Sumoylation of LIN-1 promotes transcriptional repression and inhibition of vulval cell fates

Department of Molecular Biology and Pharmacology Washington University School of Medicine, St Louis, MO 63110, USA

^* Author for correspondence (e-mail: kornfeld{at}molecool.wustl.edu)

Accepted 23 December 2004

The LIN-1 ETS transcription factor inhibits vulval cell fates during Caenorhabditis elegans development. We demonstrate that LIN-1 interacts with UBC-9, a small ubiquitin-related modifier (SUMO) conjugating enzyme. This interaction is mediated by two consensus sumoylation motifs in LIN-1. Biochemical studies showed that LIN-1 is covalently modified by SUMO-1. ubc-9 and smo-1, the gene encoding SUMO-1, inhibit vulval cell fates and function at the level of lin-1, indicating that sumoylation promotes LIN-1 inhibition of vulval cell fates. Sumoylation of LIN-1 promoted transcriptional repression and mediated an interaction with MEP-1, a protein previously shown to associate with the nucleosome remodeling and histone deacetylation (NuRD) transcriptional repression complex. Genetic studies showed that mep-1 inhibits vulval cell fates and functions at the level of lin-1. We propose that sumoylation of LIN-1 mediates an interaction with MEP-1 that contributes to transcriptional repression of genes that promote vulval cell fates. These studies identify a molecular mechanism for SUMO-mediated transcriptional repression.

Key words: LIN-1, SUMO, ETS, Chromatin, Vulval development, Transcription

This article has been cited by other articles:

				W. O. Miles, E. Jaffray, S. G. Campbell, S. Takeda, L. J. Bayston, S. P. Basu, M. Li, L. A. Raftery, M. P. Ashe, R. T. Hay, et al. Medea SUMOylation restricts the signaling range of the Dpp morphogen in the Drosophila embryo Genes & Dev., September 15, 2008; 22(18): 2578 - 2590. [Abstract] [Full Text] [PDF]

				M. C. Touz, A. S. Ropolo, M. R. Rivero, C. V. Vranych, J. T. Conrad, S. G. Svard, and T. E. Nash Arginine deiminase has multiple regulatory roles in the biology of Giardia lamblia J. Cell Sci., September 1, 2008; 121(17): 2930 - 2938. [Abstract] [Full Text] [PDF]

				M. Siatecka, L. Xue, and J. J. Bieker Sumoylation of EKLF Promotes Transcriptional Repression and Is Involved in Inhibition of Megakaryopoiesis Mol. Cell. Biol., December 15, 2007; 27(24): 8547 - 8560. [Abstract] [Full Text] [PDF]

				A. F. Deyrieux, G. Rosas-Acosta, M. A. Ozbun, and V. G. Wilson Sumoylation dynamics during keratinocyte differentiation J. Cell Sci., January 1, 2007; 120(1): 125 - 136. [Abstract] [Full Text] [PDF]

				S. M. Lauberth and M. Rauchman A Conserved 12-Amino Acid Motif in Sall1 Recruits the Nucleosome Remodeling and Deacetylase Corepressor Complex J. Biol. Chem., August 18, 2006; 281(33): 23922 - 23931. [Abstract] [Full Text] [PDF]

				M. S. Macauley, W. J. Errington, M. Scharpf, C. D. Mackereth, A. G. Blaszczak, B. J. Graves, and L. P. McIntosh Beads-on-a-String, Characterization of Ets-1 Sumoylated within Its Flexible N-terminal Sequence J. Biol. Chem., February 17, 2006; 281(7): 4164 - 4172. [Abstract] [Full Text] [PDF]

				M. V. Sundaram The love-hate relationship between Ras and Notch Genes & Dev., August 15, 2005; 19(16): 1825 - 1839. [Abstract] [Full Text] [PDF]