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First published online 23 February 2005
doi: 10.1242/dev.01697
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1 Department of Molecular Biology and Biochemistry, Osaka University Graduate
School of Medicine/Faculty of Medicine, Suita 565-0871, Japan
2 KAN Research Institute, 93 Chudoji-Awatamachi, Shimogyo-ku, Kyoto 600-8815,
Japan
3 Department of Molecular Biology, Osaka Medical Center for Cancer and
Cardiovascular Diseases, Osaka 537-8511, Japan
* Author for correspondence (e-mail: ytakai{at}molbio.med.osaka-u.ac.jp)
Accepted 14 January 2005
Nectins are Ca2+-independent immunoglobulin-like cell-cell-adhesion molecules consisting of four members. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in the testis, in cooperation with, or independently of, cadherins. To further explore the function of nectins, we generated nectin 1–/– and nectin 3–/– mice. Both nectin 1–/– and nectin 3–/– mice showed a virtually identical ocular phenotype, microphthalmia, accompanied by a separation of the apex-apex contact between the pigment and non-pigment cell layers of the ciliary epithelia. Immunofluorescence and immunoelectron microscopy revealed that nectin 1 and nectin 3, but not nectin 2, localized at the apex-apex junctions between the pigment and non-pigment cell layers of the ciliary epithelia. However, nectin 1–/– and nectin 3–/– mice showed no impairment of the apicolateral junctions between the pigment epithelia where nectin 1, nectin 2 and nectin 3 localized, or of the apicolateral junctions between the non-pigment epithelia where nectin 2 and nectin 3, but not nectin 1, localized. These results indicate that the heterophilic trans-interaction between nectin 1 and nectin 3 plays a sentinel role in establishing the apex-apex adhesion between the pigment and non-pigment cell layers of the ciliary epithelia that is essential for the morphogenesis of the ciliary body.
Key words: Nectin, Afadin, Pigment epithelia, Non-pigment epithelia, Ciliary body, Pvrl1, Pvrl3, Mouse
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