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First published online 16 March 2005
doi: 10.1242/dev.01780

This Article Figures Only Full Text Full Text (PDF) An erratum has been published All Versions of this Article: dev.01780v1 132/8/1875    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ji, J.-Y. Articles by Schubiger, G. Search for Related Content PubMed PubMed Citation Articles by Ji, J.-Y. Articles by Schubiger, G.

Genetic interactions between Cdk1-CyclinB and the Separase complex in Drosophila

Jun-Yuan Ji^*,, Justin Crest and Gerold Schubiger

Department of Biology, University of Washington, Seattle, WA 98195-1800, USA

Author for correspondence (e-mail: jji{at}partners.org)

Accepted 11 February 2005

Cdk1-CycB plays a key role in regulating many aspects of cell-cycle events, such as cytoskeletal dynamics and chromosome behavior during mitosis. To investigate how Cdk1-CycB controls the coordination of these events, we performed a dosage-sensitive genetic screen, which is based on the observations that increased maternal CycB (four extra gene copies) leads to higher Cdk1-CycB activity in early Drosophila embryos, delays anaphase onset, and generates a sensitized non-lethal phenotype at the blastoderm stage (defined as six cycB phenotype). Here, we report that mutations in the gene three rows (thr) enhance, while mutations in pimples (pim, encoding Drosophila Securin) or separase (Sse) suppress, the sensitized phenotype. In Drosophila, both Pim and Thr are known to regulate Sse activity, and activated Sse cleaves a Cohesin subunit to initiate anaphase. Compared with the six cycB embryos, reducing Thr in embryos with more CycB further delays the initiation of anaphase, whereas reducing either Pim or Sse has the opposite effect. Furthermore, nuclei move slower during cortical migration in embryos with higher Cdk1-CycB activity, whereas reducing either Pim or Sse suppresses this phenotype by causing a novel nuclear migration pattern. Therefore, our genetic screen has identified all three components of the complex that regulates sister chromatid separation, and our observations indicate that interactions between Cdk1-CycB and the Pim-Thr-Sse complex are dosage sensitive.

Key words: Cdk1-CycB, three rows, Separase, Anaphase, Drosophila

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