spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 23 March 2005
doi: 10.1242/dev.01801

Development 132, 2093-2102 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
dev.01801v1
132/9/2093    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Strumpf, D.
Right arrow Articles by Rossant, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strumpf, D.
Right arrow Articles by Rossant, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst

Dan Strumpf1, Chai-An Mao1,*, Yojiro Yamanaka1, Amy Ralston1, Kallayanee Chawengsaksophak1, Felix Beck2 and Janet Rossant1,3,{dagger}

1 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Ontario, Canada
2 Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
3 Department of Molecular and Medical Genetics, University of Toronto, Toronto M5S 1A8, Ontario, Canada

{dagger} Author for correspondence (e-mail: rossant{at}mshri.on.ca)

Accepted 22 February 2005

Blastocyst formation marks the segregation of the first two cell lineages in the mammalian preimplantation embryo: the inner cell mass (ICM) that will form the embryo proper and the trophectoderm (TE) that gives rise to the trophoblast lineage. Commitment to ICM lineage is attributed to the function of the two transcription factors, Oct4 (encoded by Pou5f1) and Nanog. However, a positive regulator of TE cell fate has not been described. The T-box protein eomesodermin (Eomes) and the caudal-type homeodomain protein Cdx2 are expressed in the TE, and both Eomes and Cdx2 homozygous mutant embryos die around the time of implantation. A block in early TE differentiation occurs in Eomes mutant blastocysts. However, Eomes mutant blastocysts implant, and Cdx2 and Oct4 expression are correctly restricted to the ICM TE. Blastocoel formation initiates in Cdx2 mutants but epithelial integrity is not maintained and embryos fail to implant. Loss of Cdx2 results in failure to downregulate Oct4 and Nanog in outer cells of the blastocyst and subsequent death of those cells. Thus, Cdx2 is essential for segregation of the ICM and TE lineages at the blastocyst stage by ensuring repression of Oct4 and Nanog in the TE.

Key words: Trophoblast, Stem cells, Pou5f1, Oct4, Eomesodermin, Nanog, Mouse


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in Development:

Cdx2: controlling early lineage segregation

Development 2005 132: e901. [Full Text]  



This article has been cited by other articles:


Home page
 Genes Dev.Home page
P. Yuan, J. Han, G. Guo, Y. L. Orlov, M. Huss, Y.-H. Loh, L.-P. Yaw, P. Robson, B. Lim, and H.-H. Ng
Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells
Genes & Dev., November 1, 2009; 23(21): 2507 - 2520.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Home, S. Ray, D. Dutta, I. Bronshteyn, M. Larson, and S. Paul
GATA3 Is Selectively Expressed in the Trophectoderm of Peri-implantation Embryo and Directly Regulates Cdx2 Gene Expression
J. Biol. Chem., October 16, 2009; 284(42): 28729 - 28737.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Nichols, J. Silva, M. Roode, and A. Smith
Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo
Development, October 1, 2009; 136(19): 3215 - 3222.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
G. C. Douglas, C. A. VandeVoort, P. Kumar, T.-C. Chang, and T. G. Golos
Trophoblast Stem Cells: Models for Investigating Trophectoderm Differentiation and Placental Development
Endocr. Rev., May 1, 2009; 30(3): 228 - 240.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Rossant and P. P. L. Tam
Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse
Development, March 1, 2009; 136(5): 701 - 713.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
T. Fujimori, Y. Kurotaki, K. Komatsu, and Y.-i. Nabeshima
Morphological Organization of the Mouse Preimplantation Embryo
Reproductive Sciences, February 1, 2009; 16(2): 171 - 177.
[Abstract] [PDF]

Home page
 JCBHome page
M. Yamamoto, H. Beppu, K. Takaoka, C. Meno, E. Li, K. Miyazono, and H. Hamada
Antagonism between Smad1 and Smad2 signaling determines the site of distal visceral endoderm formation in the mouse embryo
J. Cell Biol., January 26, 2009; 184(2): 323 - 334.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Picozzi, F. Wang, K. Cronk, and K. Ryan
Eomesodermin Requires Transforming Growth Factor-{beta}/Activin Signaling and Binds Smad2 to Activate Mesodermal Genes
J. Biol. Chem., January 23, 2009; 284(4): 2397 - 2408.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H.-T. Bui, S. Wakayama, S. Kishigami, J.-H. Kim, N. Van Thuan, and T. Wakayama
The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming
Development, December 1, 2008; 135(23): 3935 - 3945.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Goller, F. Vauti, S. Ramasamy, and H.-H. Arnold
Transcriptional Regulator BPTF/FAC1 Is Essential for Trophoblast Differentiation during Early Mouse Development
Mol. Cell. Biol., November 15, 2008; 28(22): 6819 - 6827.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. Jedrusik, D.-E. Parfitt, G. Guo, M. Skamagki, J. B. Grabarek, M. H. Johnson, P. Robson, and M. Zernicka-Goetz
Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo
Genes & Dev., October 1, 2008; 22(19): 2692 - 2706.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. Plusa, A. Piliszek, S. Frankenberg, J. Artus, and A.-K. Hadjantonakis
Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst
Development, September 15, 2008; 135(18): 3081 - 3091.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Wu, W. Zhang, G. Chen, L. Cheng, J. Liao, N. Jia, Y. Gao, H. Dai, J. Yuan, L. Cheng, et al.
Combinatorial Signals of Activin/Nodal and Bone Morphogenic Protein Regulate the Early Lineage Segregation of Human Embryonic Stem Cells
J. Biol. Chem., September 5, 2008; 283(36): 24991 - 25002.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. E. Torres-Padilla
Cell identity in the preimplantation mammalian embryo: an epigenetic perspective from the mouse
Hum. Reprod., June 1, 2008; 23(6): 1246 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
S J Kimber, S F Sneddon, D J Bloor, A M El-Bareg, J A Hawkhead, A D Metcalfe, F D Houghton, H J Leese, A Rutherford, B A Lieberman, et al.
Expression of genes involved in early cell fate decisions in human embryos and their regulation by growth factors
Reproduction, May 1, 2008; 135(5): 635 - 647.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. Honda, N. Motosugi, T. Nagai, M. Tanemura, and T. Hiiragi
Computer simulation of emerging asymmetry in the mouse blastocyst
Development, April 15, 2008; 135(8): 1407 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. J. Arnold, U. K. Hofmann, E. K. Bikoff, and E. J. Robertson
Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse
Development, February 1, 2008; 135(3): 501 - 511.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C.-A. Mao, T. Kiyama, P. Pan, Y. Furuta, A.-K. Hadjantonakis, and W. H. Klein
Eomesodermin, a target gene of Pou4f2, is required for retinal ganglion cell and optic nerve development in the mouse
Development, January 15, 2008; 135(2): 271 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Rieger, R. Poppino, R. Sheridan, K. Moley, R. Mitra, and D. Gottlieb
Polony analysis of gene expression in ES cells and blastocysts
Nucleic Acids Res., December 10, 2007; (2007) gkm1076v1.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J.-E. Dietrich and T. Hiiragi
Stochastic patterning in the mouse pre-implantation embryo
Development, December 1, 2007; 134(23): 4219 - 4231.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Yagi, M. J. Kohn, I. Karavanova, K. J. Kaneko, D. Vullhorst, M. L. DePamphilis, and A. Buonanno
Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development
Development, November 1, 2007; 134(21): 3827 - 3836.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
F. Lavial, H. Acloque, F. Bertocchini, D. J. MacLeod, S. Boast, E. Bachelard, G. Montillet, S. Thenot, H. M. Sang, C. D. Stern, et al.
The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells
Development, October 1, 2007; 134(19): 3549 - 3563.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Wuensch, F. A. Habermann, S. Kurosaka, R. Klose, V. Zakhartchenko, H.-D. Reichenbach, F. Sinowatz, K. J. McLaughlin, and E. Wolf
Quantitative Monitoring of Pluripotency Gene Activation after Somatic Cloning in Cattle
Biol Reprod, June 1, 2007; 76(6): 983 - 991.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Kurotaki, K. Hatta, K. Nakao, Y.-i. Nabeshima, and T. Fujimori
Blastocyst Axis Is Specified Independently of Early Cell Lineage But Aligns with the ZP Shape
Science, May 4, 2007; 316(5825): 719 - 723.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Q. A. Winger, J. Guttormsen, H. Gavin, and F. Bhushan
Heat Shock Protein 1 and the Mitogen-Activated Protein Kinase 14 Pathway Are Important for Mouse Trophoblast Stem Cell Differentiation
Biol Reprod, May 1, 2007; 76(5): 884 - 891.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Adachi, C. Soeta-Saneyoshi, H. Sagara, and Y. Iwakura
Crucial Role of Bysl in Mammalian Preimplantation Development as an Integral Factor for 40S Ribosome Biogenesis
Mol. Cell. Biol., March 15, 2007; 27(6): 2202 - 2214.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Kaji, J. Nichols, and B. Hendrich
Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells
Development, March 15, 2007; 134(6): 1123 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
U. Elling, C. Klasen, T. Eisenberger, K. Anlag, and M. Treier
Murine inner cell mass-derived lineages depend on Sall4 function
PNAS, October 31, 2006; 103(44): 16319 - 16324.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Pereira, F. Yi, and B. J. Merrill
Repression of Nanog Gene Transcription by Tcf3 Limits Embryonic Stem Cell Self-Renewal
Mol. Cell. Biol., October 15, 2006; 26(20): 7479 - 7491.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Y. Choi, A. I. Romer, M. Hu, M. Lepourcelet, A. Mechoor, A. Yesilaltay, M. Krieger, P. A. Gray, and R. A. Shivdasani
A dynamic expression survey identifies transcription factors relevant in mouse digestive tract development
Development, October 15, 2006; 133(20): 4119 - 4129.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. A. Gonzalez, K.-e K. Tachibana, D. J. Adams, L. van der Weyden, M. Hemberger, N. Coleman, A. Bradley, and R. A. Laskey
Geminin is essential to prevent endoreduplication and to form pluripotent cells during mammalian development
Genes & Dev., July 15, 2006; 20(14): 1880 - 1884.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R. Harun, L. Ruban, M. Matin, J. Draper, N.M. Jenkins, G.C. Liew, P.W. Andrews, T.C. Li, S.M. Laird, and H.D.M. Moore
Cytotrophoblast stem cell lines derived from human embryonic stem cells and their capacity to mimic invasive implantation events
Hum. Reprod., June 1, 2006; 21(6): 1349 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Cormier, S. Le Bras, C. Souilhol, S. Vandormael-Pournin, B. Durand, C. Babinet, P. Baldacci, and M. Cohen-Tannoudji
The Murine Ortholog of Notchless, a Direct Regulator of the Notch Pathway in Drosophila melanogaster, Is Essential for Survival of Inner Cell Mass Cells.
Mol. Cell. Biol., May 1, 2006; 26(9): 3541 - 3549.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. Huntriss, M. Hinkins, and H.M. Picton
cDNA cloning and expression of the human NOBOX gene in oocytes and ovarian follicles
Mol. Hum. Reprod., May 1, 2006; 12(5): 283 - 289.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Jouneau, Q. Zhou, A. Camus, V. Brochard, L. Maulny, J. Collignon, and J.-P. Renard
Developmental abnormalities of NT mouse embryos appear early after implantation.
Development, April 1, 2006; 133(8): 1597 - 1607.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
K. Deb, M. Sivaguru, H. Y. Yong, and R. M. Roberts
Cdx2 Gene Expression and Trophectoderm Lineage Specification in Mouse Embryos
Science, February 17, 2006; 311(5763): 992 - 996.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. van Nes, W. de Graaff, F. Lebrin, M. Gerhard, F. Beck, and J. Deschamps
The Cdx4 mutation affects axial development and reveals an essential role of Cdx genes in the ontogenesis of the placental labyrinth in mice
Development, February 1, 2006; 133(3): 419 - 428.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Deschamps and J. van Nes
Developmental regulation of the Hox genes during axial morphogenesis in the mouse
Development, July 1, 2005; 132(13): 2931 - 2942.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005