QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 3 May 2006
doi: 10.1242/dev.02386

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: dev.02386v1 133/11/2177    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gupta, S. Articles by Evans, T. Search for Related Content PubMed PubMed Citation Articles by Gupta, S. Articles by Evans, T.

BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis

¹ Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
² Division of Hematology/Oncology, Children's Hospital of Boston, Department of Pediatrics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.

^* Author for correspondence (e-mail: tevans{at}aecom.yu.edu)

Accepted 31 March 2006

The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.

Key words: Hematopoiesis, Endothelial, Pronephros, Transgenic, Zebrafish, lmo2

This article has been cited by other articles:

				Y. Zhang, X.-T. Bai, K.-Y. Zhu, Y. Jin, M. Deng, H.-Y. Le, Y.-F. Fu, Y. Chen, J. Zhu, A. T. Look, et al. In Vivo Interstitial Migration of Primitive Macrophages Mediated by JNK-Matrix Metalloproteinase 13 Signaling in Response to Acute Injury J. Immunol., August 1, 2008; 181(3): 2155 - 2164. [Abstract] [Full Text] [PDF]

				H.-Y. Le, Y. Zhang, H. Liu, L.-H. Ma, Y. Jin, Q.-H. Huang, Y. Chen, M. Deng, Z. Chen, S.-J. Chen, et al. eena Promotes Myeloid Proliferation through Stimulating ERK1/2 Phosphorylation in Zebrafish J. Biol. Chem., June 20, 2008; 283(25): 17652 - 17661. [Abstract] [Full Text] [PDF]

				F. Lohmann and J. J. Bieker Activation of Eklf expression during hematopoiesis by Gata2 and Smad5 prior to erythroid commitment Development, June 15, 2008; 135(12): 2071 - 2082. [Abstract] [Full Text] [PDF]

				L. J. McReynolds, S. Gupta, M. E. Figueroa, M. C. Mullins, and T. Evans Smad1 and Smad5 differentially regulate embryonic hematopoiesis Blood, December 1, 2007; 110(12): 3881 - 3890. [Abstract] [Full Text] [PDF]

				J. E. Pimanda, I. J. Donaldson, M. F. T. R. de Bruijn, S. Kinston, K. Knezevic, L. Huckle, S. Piltz, J.-R. Landry, A. R. Green, D. Tannahill, et al. The SCL transcriptional network and BMP signaling pathway interact to regulate RUNX1 activity PNAS, January 16, 2007; 104(3): 840 - 845. [Abstract] [Full Text] [PDF]

				A. T. Naito, I. Shiojima, H. Akazawa, K. Hidaka, T. Morisaki, A. Kikuchi, and I. Komuro Developmental stage-specific biphasic roles of Wnt/beta-catenin signaling in cardiomyogenesis and hematopoiesis PNAS, December 26, 2006; 103(52): 19812 - 19817. [Abstract] [Full Text] [PDF]