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First published online 3 July 2006
doi: 10.1242/dev.02443
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1 Department of Dermatology, Leiden University Medical Center (LUMC), Leiden,
The Netherlands.
2 Keratinocyte Laboratory, Cancer Research UK, London Research Institute,
London, UK.
3 Laboratory for Lymphocyte Development, RIKEN Research Center for Allergy and
Immunology, Yokohama, Japan.
4 Department of Immunology, Central and Eastern Clinical School, Monash
University, Melbourne, Australia.
5 Department of Toxicogenetics, LUMC, Leiden, The Netherlands.
6 Department of Immunology and the Center for Electron Microscopy/Department of
Molecular and Cellular Biology, LUMC, Leiden, The Netherlands.
Author for correspondence (e-mail:
vanewijk{at}lumc.nl)
Accepted 15 May 2006
We describe a novel murine progenitor cell population localised to a
previously uncharacterised region between sebaceous glands and the hair
follicle bulge, defined by its reactivity to the thymic epithelial progenitor
cell marker MTS24. MTS24 labels a membrane-bound antigen present during the
early stages of hair follicle development and in adult mice. MTS24
co-localises with expression of 
6-integrin and keratin 14, indicating
that these cells include basal keratinocytes. This novel population does not
express the bulge-specific stem cell markers CD34 or keratin 15, and is
infrequently BrdU label retaining. MTS24-positive and -negative keratinocyte
populations were isolated by flow cytometry and assessed for colony-forming
efficiency. MTS24-positive keratinocytes exhibited a two-fold increase in
colony formation and colony size compared to MTS24-negative basal
keratinocytes. In addition, both the MTS24-positive and CD34-positive
subpopulations were capable of producing secondary colonies after serial
passage of individual cell clones. Finally, gene expression profiles of MTS24
and CD34 subpopulations were compared. These results showed that the overall
gene expression profile of MTS24-positive cells resembles the pattern
previously reported in bulge stem cells. Taken together, these data suggest
that the cell-surface marker MTS24 identifies a new reservoir of hair follicle
keratinocytes with a proliferative capacity and gene expression profile
suggestive of progenitor or stem cells.
Key words: Hair follicle, Progenitor cell, Stem cell, Epidermis, Keratinocyte, Mouse
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