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First published online 3 July 2006
doi: 10.1242/dev.02443

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The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells

Joanne G. W. Nijhof^1,*, Kristin M. Braun^2,*,, Adam Giangreco², Carina van Pelt¹, Hiroshi Kawamoto³, Richard L. Boyd⁴, Rein Willemze¹, Leon H. F. Mullenders⁵, Fiona M. Watt², Frank R. de Gruijl¹ and Willem van Ewijk^6,

¹ Department of Dermatology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
² Keratinocyte Laboratory, Cancer Research UK, London Research Institute, London, UK.
³ Laboratory for Lymphocyte Development, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan.
⁴ Department of Immunology, Central and Eastern Clinical School, Monash University, Melbourne, Australia.
⁵ Department of Toxicogenetics, LUMC, Leiden, The Netherlands.
⁶ Department of Immunology and the Center for Electron Microscopy/Department of Molecular and Cellular Biology, LUMC, Leiden, The Netherlands.

Author for correspondence (e-mail: vanewijk{at}lumc.nl)

Accepted 15 May 2006

We describe a novel murine progenitor cell population localised to a previously uncharacterised region between sebaceous glands and the hair follicle bulge, defined by its reactivity to the thymic epithelial progenitor cell marker MTS24. MTS24 labels a membrane-bound antigen present during the early stages of hair follicle development and in adult mice. MTS24 co-localises with expression of 6-integrin and keratin 14, indicating that these cells include basal keratinocytes. This novel population does not express the bulge-specific stem cell markers CD34 or keratin 15, and is infrequently BrdU label retaining. MTS24-positive and -negative keratinocyte populations were isolated by flow cytometry and assessed for colony-forming efficiency. MTS24-positive keratinocytes exhibited a two-fold increase in colony formation and colony size compared to MTS24-negative basal keratinocytes. In addition, both the MTS24-positive and CD34-positive subpopulations were capable of producing secondary colonies after serial passage of individual cell clones. Finally, gene expression profiles of MTS24 and CD34 subpopulations were compared. These results showed that the overall gene expression profile of MTS24-positive cells resembles the pattern previously reported in bulge stem cells. Taken together, these data suggest that the cell-surface marker MTS24 identifies a new reservoir of hair follicle keratinocytes with a proliferative capacity and gene expression profile suggestive of progenitor or stem cells.

Key words: Hair follicle, Progenitor cell, Stem cell, Epidermis, Keratinocyte, Mouse

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