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First published online 16 August 2006
doi: 10.1242/dev.02541

Development 133, 3671-3681 (2006)
Published by The Company of Biologists 2006
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Derivation and large-scale expansion of multipotent astroglial neural progenitors from adult human brain

Noah M. Walton1, Benjamin M. Sutter2, Huan-Xin Chen3, Lung-Ji Chang4, Steven N. Roper1,4, Bjorn Scheffler1,* and Dennis A. Steindler1,2,4,5,*,{dagger}

1 Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.
2 Shands Cancer Center, University of Florida, Gainesville, FL 32610, USA.
3 Department of Neurosurgery, University of Florida, Gainesville, FL 32610, USA.
4 Department of Molecular Genetics and Microbiology University of Florida, Gainesville, FL 32610, USA.
5 Program in Stem Cell Biology and Regenerative Medicine, University of Florida, Gainesville, FL 32610, USA.

{dagger} Author for correspondence (e-mail: steindler{at}mbi.ufl.edu)

Accepted 6 July 2006

The isolation and expansion of human neural cell types has become increasingly relevant in restorative neurobiology. Although embryonic and fetal tissue are frequently envisaged as providing sufficiently primordial cells for such applications, the developmental plasticity of endogenous adult neural cells remains largely unclear. To examine the developmental potential of adult human brain cells, we applied conditions favoring the growth of neural stem cells to multiple cortical regions, resulting in the identification and selection of a population of adult human neural progenitors (AHNPs). These nestin+ progenitors may be derived from multiple forebrain regions, are maintainable in adherent conditions, co-express multiple glial and immature markers, and are highly expandable, allowing a single progenitor to theoretically form sufficient cells for ~4x107 adult brains. AHNPs longitudinally maintain the ability to generate both glial and neuronal cell types in vivo and in vitro, and are amenable to genetic modification and transplantation. These findings suggest an unprecedented degree of inducible plasticity is retained by cells of the adult central nervous system.

Key words: Astrocyte, Progenitor, Plasticity, Human, Expansion, Commitment, Transplantation


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Developmental plasticity of adult human brain revealed

Development 2006 133: e1803. [Full Text]  





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