spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 20 September 2006
doi: 10.1242/dev.02566

Development 133, 4085-4096 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrowOA All Versions of this Article:
dev.02566v1
133/20/4085    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanabe, K.
Right arrow Articles by Nakagawa, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanabe, K.
Right arrow Articles by Nakagawa, S.

Cadherin is required for dendritic morphogenesis and synaptic terminal organization of retinal horizontal cells

Koji Tanabe1,2,3, Yoshiko Takahashi1,4, Yuki Sato1,4, Koichi Kawakami5, Masatoshi Takeichi1,2 and Shinichi Nakagawa1,3,*

1 RIKEN Center for Developmental Biology, 2-2-3 Minatojima Minamimachi, Chuoku, Kobe 650-0047, Japan.
2 Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
3 Nakagawa Initiative Research Unit, RIKEN Frontier Research Program, 2-1 Hirosawa, Wako 351-0198, Japan.
4 Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, NARA, 630-0192, Japan.
5 Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan.

* Author for correspondence (e-mail: nakagawas{at}riken.jp)

Accepted 4 August 2006

Dendrite morphology of neurons provides a structural basis for their physiological characteristics, and is precisely regulated in a cell type-dependent manner. Using a unique transposon-mediated gene transfer system that enables conditional and cell-type specific expression of exogenous genes, we investigated the role of cadherin on dendritic morphogenesis of horizontal cells in the developing chicken retina. We first visualized single horizontal cells by overexpressing membrane-targeted EGFP, and confirmed that there were three subtypes of horizontal cells, the dendritic terminals of which projected to distinct synaptic sites in the outer plexiform layer. Expression of a dominant-negative cadherin decreased the dendritic field size, and perturbed the termination of dendritic processes onto the photoreceptor cells. The cadherin blockade also impaired the accumulation of GluR4, a postsynaptic marker, at the cone pedicles. We thus provide in vivo evidence that cadherin is required for dendrite morphogenesis of horizontal cells and subsequent synapse formation with photoreceptor cells in the vertebrate retina.

Key words: Horizontal cell, Retina, Cadherin, Dendrite, Transposon, Chicken




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. B. Rompani and C. L. Cepko
Retinal progenitor cells can produce restricted subsets of horizontal cells
PNAS, January 8, 2008; 105(1): 192 - 197.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006