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First published online 1 November 2006
doi: 10.1242/dev.02650

Development 133, 4655-4665 (2006)
Published by The Company of Biologists 2006
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Bchs, a BEACH domain protein, antagonizes Rab11 in synapse morphogenesis and other developmental events

Rita Khodosh1,2, Adela Augsburger1, Thomas L. Schwarz2 and Paul A. Garrity1,3,*

1 Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue 68-230B, Cambridge, MA 02139, USA.
2 Division of Neuroscience, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
3 Biology Department, Brandeis University, MS-008, 415 South Street, Waltham, MA 02454, USA.

* Author for correspondence (e-mail: pgarrity{at}brandeis.edu)

Accepted 18 September 2006

BEACH proteins, an evolutionarily conserved family characterized by the presence of a BEACH (Beige and Chédiak-Higashi) domain, have been implicated in membrane trafficking, but how they interact with the membrane trafficking machinery is unknown. Here we show that the Drosophila BEACH protein Bchs (Blue cheese) acts during development as an antagonist of Rab11, a small GTPase involved in vesicle trafficking. We find that reduction in, or loss of, bchs function restores viability and normal bristle development in animals with reduced rab11 function, while reductions in rab11 function exacerbate defects caused by bchs overexpression in the eye. Consistent with a role for Bchs in modulating Rab11-dependent trafficking, Bchs protein is associated with vesicles and extensively colocalized with Rab11 at the neuromuscular junction (NMJ). At the NMJ, we find that rab11 is important for synaptic morphogenesis, as reductions in rab11 function cause increases in bouton density and branching. These defects are also suppressed by loss of bchs. Taken together, these data identify Bchs as an antagonist of Rab11 during development and uncover a role for these regulators of vesicle trafficking in synaptic morphogenesis. This raises the interesting possibility that Bchs and other BEACH proteins may regulate vesicle traffic via interactions with Rab GTPases.

Key words: BEACH domain, Vesicle trafficking, Neuromuscular junction, Bouton, Bristle development, Drosophila


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Development 2006 133: e2302. [Full Text]  



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© The Company of Biologists Ltd 2006