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First published online January 25, 2006
doi: 10.1242/10.1242/dev.02226
1 CNRS URA 2578, Department of Developmental Biology, Pasteur Institute, 28 rue
du Dr Roux, 75 724 Paris Cedex 15, France.
2 Stem Cell Research Institute, Dibit, H.S. Raffaele, Via Olgettina 58, 20132
Milano, Italy.
3 Department of Biology, University of Milan, Via Celoria 26, 20133 Milano,
Italy.
4 Institute of Cell Biology and Tissue Engineering, San Raffaele Biomedical
Science Park of Rome, Via Castel Romano 100/2, 00128 Rome, Italy.
Author for correspondence (e-mail:
margab{at}pasteur.fr)
Accepted 14 December 2005
We show that cells of the dorsal aorta, an early blood vessel, and of the
myotome, the first skeletal muscle to form within the somite, derive from a
common progenitor in the mouse embryo. This conclusion is based on a
retrospective clonal analysis, using a nlaacZ reporter targeted to
the 
-cardiac actin gene. A rare intragenic recombination event results
in a functional nlacZ sequence, giving rise to clones of
ß-galactosidase-positive cells. Periendothelial and vascular smooth
muscle cells of the dorsal aorta are the main cell types labelled,
demonstrating that these are clonally related to the paraxial mesoderm-derived
cells of skeletal muscle. Rare endothelial cells are also seen in some clones.
In younger clones, arising from a recent recombination event, myotomal
labelling is predominantly in the hypaxial somite, adjacent to labelled smooth
muscle cells in the aorta. Analysis of Pax3GFP/+ embryos
shows that these cells are Pax3 negative but GFP positive, with fluorescent
cells in the intervening region between the aorta and the somite. This is
consistent with the direct migration of smooth muscle precursor cells that had
expressed Pax3. These results are discussed in terms of the paraxial mesoderm
contribution to the aorta and of the mesoangioblast stem cells that derive
from it.
Key words: Dorsal aorta, Skeletal muscle, Smooth muscle, Clonal analysis, LaacZ, Pax3
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