|
|
|
|||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 8 March 2006
doi: 10.1242/dev.02315
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

1 Center of Experimental Medicine, Institute of Anatomy I, University Hospital
Hamburg-Eppendorf, Hamburg, Germany.
2 Department of Cardiac Surgery, University Hospital Hamburg-Eppendorf, Hamburg,
Germany,
3 Department of Hematology and Oncology, University Hospital Hamburg-Eppendorf,
Hamburg, Germany.
4 Department of Cardiology, University Hospital Hamburg-Eppendorf, Hamburg,
Germany.
5 Department of Internal Medicine, University of Schleswig-Holstein, Campus
Lübeck, Germany.
Author for correspondence (e-mail:
erguen{at}uke.uni-hamburg.de)
Accepted 8 February 2006
Here, we report the existence of endothelial precursor (EPC) and stem cells in a distinct zone of the vascular wall that are capable to differentiate into mature endothelial cells, hematopoietic and local immune cells, such as macrophages. This zone has been identified to be localized between smooth muscle and adventitial layer of human adult vascular wall. It predominantly contains CD34-positive (+) but CD31-negative (-) cells, which also express VEGFR2 and TIE2. Only few cells in this zone of the vascular wall are positive for CD45. In a ring assay using the fragments of human internal thoracic artery (HITA), we show here that the CD34+ cells of the HITA-wall form capillary sprouts ex vivo and are apparently recruited for capillary formation by tumor cells. New vessels formed by these vascular wall resident EPCs express markers for angiogenically activated endothelial cells, such as CEACAM1, and also for mature endothelial cells, such as VE-cadherin or occludin. Vascular wall areas containing EPCs are found in large and middle sized arteries and veins of all organs studied here. These data suggest the existence of a `vasculogenic zone' in the wall of adult human blood vessels, which may serve as a source for progenitor cells for postnatal vasculogenesis, contributing to tumor vascularization and local immune response.
Key words: Rat, Vasculogenesis, VEGFR
This article has been cited by other articles:
![]() |
K. Iohara, L. Zheng, H. Wake, M. Ito, J. Nabekura, H. Wakita, H. Nakamura, T. Into, K. Matsushita, and M. Nakashima A Novel Stem Cell Source for Vasculogenesis in Ischemia: Subfraction of Side Population Cells from Dental Pulp Stem Cells, September 1, 2008; 26(9): 2408 - 2418. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. N. Passman, X. R. Dong, S.-P. Wu, C. T. Maguire, K. A. Hogan, V. L. Bautch, and M. W. Majesky A sonic hedgehog signaling domain in the arterial adventitia supports resident Sca1+ smooth muscle progenitor cells PNAS, July 8, 2008; 105(27): 9349 - 9354. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Zampetaki, J. P. Kirton, and Q. Xu Vascular repair by endothelial progenitor cells Cardiovasc Res, June 1, 2008; 78(3): 413 - 421. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Coveney, J. Cool, T. Oliver, and B. Capel From the Cover: Four-dimensional analysis of vascularization during primary development of an organ, the gonad PNAS, May 20, 2008; 105(20): 7212 - 7217. [Abstract] [Full Text] [PDF] |
||||
![]() |
Q. Xu Stem Cells and Transplant Arteriosclerosis Circ. Res., May 9, 2008; 102(9): 1011 - 1024. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. F. Alvarez, L. Huang, J. A. King, M. K. ElZarrad, M. C. Yoder, and T. Stevens Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L419 - L430. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Rienstra, C. J. Zeebregts, and J.-L. Hillebrands The Source of Neointimal Cells in Vein Grafts: Does the Origin Matter? Am. J. Pathol., March 1, 2008; 172(3): 566 - 570. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Kilic, L. Oliveira-Ferrer, S. Neshat-Vahid, S. Irmak, K. Obst-Pernberg, J.-H. Wurmbach, S. Loges, E. Kilic, J. Weil, H. Lauke, et al. Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1 Blood, December 15, 2007; 110(13): 4223 - 4233. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Aicher and C. Heeschen Nonbone Marrow Derived Endothelial Progenitor Cells: What Is Their Exact Location? Circ. Res., October 26, 2007; 101(9): e102 - e102. [Full Text] [PDF] |
||||
![]() |
S. Ergun and U. M. Gehling Nonbone Marrow-Derived Endothelial Progenitor Cells: What Is Their Exact Location? Circ. Res., August 3, 2007; 101(3): e31 - e31. [Full Text] [PDF] |
||||
![]() |
G. Pasquinelli, P. L. Tazzari, C. Vaselli, L. Foroni, M. Buzzi, G. Storci, F. Alviano, F. Ricci, M. Bonafe, C. Orrico, et al. Thoracic Aortas from Multiorgan Donors Are Suitable for Obtaining Resident Angiogenic Mesenchymal Stromal Cells Stem Cells, July 1, 2007; 25(7): 1627 - 1634. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Invernici, C. Emanueli, P. Madeddu, S. Cristini, S. Gadau, A. Benetti, E. Ciusani, G. Stassi, M. Siragusa, R. Nicosia, et al. Human Fetal Aorta Contains Vascular Progenitor Cells Capable of Inducing Vasculogenesis, Angiogenesis, and Myogenesis in Vitro and in a Murine Model of Peripheral Ischemia Am. J. Pathol., June 1, 2007; 170(6): 1879 - 1892. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Konishi, K. Sydow, and J. P. Cooke Dimethylarginine Dimethylaminohydrolase Promotes Endothelial Repair After Vascular Injury J. Am. Coll. Cardiol., March 13, 2007; 49(10): 1099 - 1105. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Witkowski and J. M. Hagberg Progenitor cells and age: can we fight aging with exercise? J Appl Physiol, March 1, 2007; 102(3): 834 - 835. [Full Text] [PDF] |
||||